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Kandahlawi

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    Malaysia

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  • Occupation
    Medical officer working in blood bank in Malaysia. Looking forward to gain knowledge in transfusion medicine field

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  1. Kandahlawi

    Return of used blood

    I want to do a survey regarding the practice of return used blood bags to blood bank. Currently my hospital make it compulsory to return all transfused blood bags to blood bank. Then we’ll keep it in a dedicated fridge before discard. The main reason is for investigation of delayed hemolytic transfusion reaction should it occur. But it is a very rare incidence, where we need to retrieve the segment from used blood bag for investigation (actually never encounter before). What is your hospitals practice? TQ in advance.
  2. It is anti-c. In my country, hospital blood bank still do the blood procurement, not by agency like Red Cross etc, so getting the whole blood is not a problem. We usually standby few pints of whole blood in our inventory.
  3. If I have a case needed Exchange Transfusion, both mother and baby blood group are A. How much plasma should i remove from whole blood to make the final haematocrit around 50? Is there any special formula? I want to refrain myself from using Reconstituted blood as this may expose to 2 donors.
  4. Hi, if the clinician request additional unit of red cells from type & screen sample within 72 hour period (patient already transfused), do you: 1. Crossmatch using existing T&S sample 2. Request new sample, repeat T&S, then crossmatch using new sample to make it clear, i give you all case scenario: 1.1.2016: T&S sample send 2.1.2016: request 1 unit of red cells then transfused 3.1.2016: request another unit of red cells. So in this junction, do you just crossmatch using existing sample or request new sample then do all over again. My concern is for patient who already has antibody, but undiagnosed before and undetected during current admission (antibody strength deteriorated over time), once expose to corresponding antigen, may rapidly produce antibody via secondary immune response. How rapid? I dont know tq in advance
  5. Kandahlawi

    Preparing red cell suspension for grouping

    Sorry, duplicate thread. Please delete.
  6. Hi, just wanna ask your practice in preparing red cells suspension for routine ABO blood grouping (pretransfusion testing) by tube method. In our SOP (reference: AABB Technical Method), we must wash the red cells 3 times before preparing the suspension. But in practice, we actually skip the wash step, and straight away proceed to suspense the cell in saline. We feel the wash step is a bit tedious. What do you all think of this practice?
  7. Hi, just wanna ask your practice in preparing red cells suspension for routine ABO blood grouping (pretransfusion testing) by tube method. In our SOP (reference: AABB Technical Method), we must wash the red cells 3 times before preparing the suspension. But in practice, we actually skip the wash step, and straight away proceed to suspense the cell in saline. We feel the wash step is a bit tedious. What do you all think of this practice?
  8. Kandahlawi

    DCT+ Donor

    Thanks for the responses. I read in one article, the incidence of DCT positive among donor is only around 1 in 1000 to 14,000 depend on the specificity of the methods used..i think its about time for my country to omit AHG crossmatch in the procedure..the other argument is that the reagent/screening panel cells is of caucasian origin, so we might missed out some low frequency antigen that are prevalence in our population but not in among caucasian..but again, form our experience, such case is extremely rare. Usually if antibody screening is negative, the AHG crossmatch will be mostly compatible, except in very small percentages will give AHG crossmatch incompatible (and almost all of it are due to DCT positive donor)..
  9. Kandahlawi

    DCT+ Donor

    Hi, there are 2 case scenario 1. In my country, we still do AHG crossmatch in all requested blood even though the antibody screening is negative/negative history of antibodies. Just wonder, if you do IS / electronic crossmatch only, there is risk that you might missed out DCT+ donor that only can be detected by AHG crossmatch. 2. We provide 4 unit of O RhD+ Pack red cells to be keep at Emergency Department to be used in life threatening bleeding. We will do retrospective crossmatch (AHG phase) once they use the blood using the segment from used blood bags and patients pretransfusion sample. Therefore, we routinely check DCT to each unit before issued, to ensure there is no problem with the AHG crossmatch later on. What is your comment? Thanks
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