sona Posted May 9, 2011 Share Posted May 9, 2011 dear all, heres a problem we at our blood bank did a blood group and found results anti A (NEG) ANTI B(NEG) ANTI D(POS) in reverse A CELLS (NEG) B CELLS (POS) O CELLS (NEG) . however we saw some very minute agglitinants in anti A which however disapperared after some seconds patients age was 65 and on asking he received no transfusion in the past please give the likelihoods thanx Link to comment Share on other sites More sharing options...
David Saikin Posted May 9, 2011 Share Posted May 9, 2011 might be a weak subgroup of A or the patient has a low titer/negative anti-A. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted May 9, 2011 Share Posted May 9, 2011 There are two things that spring to mind.Firstly, that he has a very weak A subgroup. You can check this either by performing adsorption and elution studies with anti-A and his red cells, or by testing his saliva for group A substance (if he happens to be a Secretor - do his Lewis typing - if he is Le(a-b+), he is a Secretor, if he is Le(a+b-), he is a non-Secretor, if he is Le(a-b-), he is an awkward customer, because he could be a Secretor or a non-Secretor).The other thing is, did you ask him if he had ever had a Bone Marrow or Stem Cell Transplant (unlikely, if he has never had a transfusion). Very often though, group A recipients of group O marrow or stem cells do not make a strong anti-A, if at all, because either they tolerate the A antigen, or they secrete A substance that inhibits the donor cell's anti-A (or both). Link to comment Share on other sites More sharing options...
John C. Staley Posted May 10, 2011 Share Posted May 10, 2011 Some patients, as they get older, simply have a reduction in their levels of natural Anti-A and Anti-B. While 65 is no where near as old as it used to be this could be the case. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted May 10, 2011 Share Posted May 10, 2011 I couldn't agree more with you John. Link to comment Share on other sites More sharing options...
EDibble Posted May 13, 2011 Share Posted May 13, 2011 How strong was the reaction with the B cells? Did you let the reverse typing cells incubate at RT for 5 to 10 minutes? Sometimes that will allow a weak backtype to show up. Link to comment Share on other sites More sharing options...
mboco Posted May 29, 2011 Share Posted May 29, 2011 might be a weak subgroup of A or the patient has a low titer/negative anti-A.probably right Mr. David. Link to comment Share on other sites More sharing options...
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