Posted April 30, 20214 yr comment_81944 Do you require a reverse type be 2+ or greater to be considered a valid type? I am not aware of any requirement for a specific grade of reaction for a reverse to be valid. If the reaction is macroscopic I have proceeded with my interpretation without further enhancement. There is a hospital within our affiliation that has more stringent rules and I want to make sure I am not missing something.
May 1, 20214 yr comment_81950 We require the reverse type to be 2+ or stronger in tube, because weaker reaction can mean subgroup or weak immunoglobulin.
May 1, 20214 yr comment_81951 That's a new one to me. I'm with Kelly, positive is positive as long as it matches the forward type. If not, let the investigation begin!
May 2, 20214 yr comment_81952 I would just be a bit suspicious if say there was a + reaction with A1 cells and 4+ with B cells in an apparent group O - or vice versa; or very weak reactions in a young healthy adult.....a bit of common sense required, that's all Edited May 2, 20214 yr by galvania clarification
May 3, 20214 yr comment_81954 I'm with galvania, we have identified a lot of subgroup patients based on the weaker reverse reactions. Such as A2,A3, Ax, Ael, Aend, and the same the Bsubgroup Edited May 3, 20214 yr by yan xia
May 3, 20214 yr comment_81955 "2.7.6 Ael Under usual conditions Ael cells are not agglutinated by anti-A or -A,B, although they do bind these anti bodies, as demonstrated by adsorption and elution [298,337–339]. Saliva from Ael secretors contains H, but no A substance. Serum from Ael individuals usually contains anti-A1 and may also contain an anti body that agglutinates A2 cells [337,339]. No A transferase has been detected in Ael serum or red cell membranes [232,287–289]. Serum H-transferase is weaker than that found in A1 or A2 serum [289]. No example of Ael was found in testing 150000 French blood donors [283], but fifive were found among 400000 Chinese from Taiwan [328]. Ael appears to be inherited as a rare gene at the ABO locus [337–339]. As a result of allelic enhancement (Section 2.10.2), AelB cells may be weakly agglutinated by some mono clonal anti-A and may resemble B(A) phenotype [340]" The text above comes from Danels' HUMAN BLOOD GROUPS the second edition, page 36 If we just look at the forward group , we may identify it as type O, but because it has weaker anti-A, we do think it is not a normal reaction ,so we will do further investigation ,and then find out it has weak A antigens. It is important for transfusion and organ transplantation. Edited May 3, 20214 yr by yan xia typo
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