Posted December 15, 20204 yr comment_81333 Hi Blood Bankers, I have a strange question, is it possible to have an anti-Jka that does not react in a ficin panel?? If so, why? Any input on this is greatly appreciated! Thank you!
December 15, 20204 yr comment_81335 Short answer: Yes How was the anti-Jka detected initially, i.e., what technique ? Assuming it was an antiglobulin test, but was it Gel, Solid Phase, LISS, PEG ? Yes, the panel cells are now ficin-treated and antibodies to Jka should be enhanced, but the test conditions that were used in the original assay may not exist when ficin-treated cells are used - one may need to add LISS or PEG, one may need to test by Gel or Solid Phase.
December 15, 20204 yr comment_81336 Couple of more strange questions, how sure are you that it is anti-Jka? If you are sure, why were you doing a ficin panel? Can't wait to see what Malcolm has to say.
December 15, 20204 yr comment_81337 I agree with exlimey. I once had an anti-E that reacted by LISS IAT, but try as we might, we could never get it to react with papain-treated red cells. We doubted the specificity, and so we sent it to Joyce Poole at the International Blood Group Reference Laboratory to have a look at it, and she confirmed both the specificity and the fact that it was non-reactive with papain-treated red cells. Anything is possible, as the antibodies refuse to read the damned text books! In the UK, it is almost a sine qua non that an "enzyme panel" is put up simultaneously with the IAT panel John, so I would almost certainly have done the same as DCeDCe. Edited December 15, 20204 yr by Malcolm Needs Spelling - or lack of!
December 16, 20204 yr comment_81341 23 hours ago, John C. Staley said: Couple of more strange questions, how sure are you that it is anti-Jka? If you are sure, why were you doing a ficin panel? Can't wait to see what Malcolm has to say. An EXCELLENT question, John, but as Malcolm suggests, is appears that there is still a large dose of "we always do that" in many laboratories.
December 16, 20204 yr comment_81345 2 hours ago, exlimey said: An EXCELLENT question, John, but as Malcolm suggests, is appears that there is still a large dose of "we always do that" in many laboratories. Ah, but don't forget that, not only did I work in a Reference Laboratory, but am also a self-confessed pedant, and so, if a patient was both c Negative and E Negative, and had an anti-c, we would go to the ends of the Earth to prove the presence or absence of an anti-E, as a matter of pride!
December 16, 20204 yr comment_81346 1 hour ago, Malcolm Needs said: Ah, but don't forget that, not only did I work in a Reference Laboratory, but am also a self-confessed pedant, and so, if a patient was both c Negative and E Negative, and had an anti-c, we would go to the ends of the Earth to prove the presence or absence of an anti-E, as a matter of pride! I confess: I'm much the same way in the OCD sense. I'm also DCeDCe (R1R1), so perhaps this issue hits a little too close to the sensitive area.
December 16, 20204 yr comment_81348 1 hour ago, exlimey said: I confess: I'm much the same way in the OCD sense. I'm also DCeDCe (R1R1), so perhaps this issue hits a little too close to the sensitive area. I, too, am DCe/DCe (R1R1). This is getting spooky! Maybe you are on to something exlimey, and should write it up for Vox Sanguinis, Transfusion and/or Transfusion Medicine, or maybe even Nature!
December 17, 20204 yr comment_81353 17 hours ago, Malcolm Needs said: I, too, am DCe/DCe (R1R1). This is getting spooky! Maybe you are on to something exlimey, and should write it up for Vox Sanguinis, Transfusion and/or Transfusion Medicine, or maybe even Nature! Thankfully I'm R1R2!!!!
December 17, 20204 yr comment_81354 18 hours ago, Malcolm Needs said: I, too, am DCe/DCe (R1R1). This is getting spooky! Maybe you are on to something exlimey, and should write it up for Vox Sanguinis, Transfusion and/or Transfusion Medicine, or maybe even Nature! An interesting idea, but I'm not sure I want my psychological dirty laundry hung out for the world to see.
December 17, 20204 yr comment_81355 1 hour ago, John C. Staley said: Thankfully I'm R1R2!!!! Sorry, John. You'll have to start your own club. Perhaps "f-negatives Rule", or something like that.
December 17, 20204 yr comment_81356 1 hour ago, John C. Staley said: Thankfully I'm R1R2!!!! Me too John
December 17, 20204 yr comment_81357 1 hour ago, exlimey said: Sorry, John. You'll have to start your own club. Perhaps "f-negatives Rule", or something like that. But don't forget that those of us who are DCe/DCe are ALSO f Negative!
December 17, 20204 yr comment_81358 10 minutes ago, Malcolm Needs said: But don't forget that those of us who are DCe/DCe are ALSO f Negative! Good point, but we're very unlikely to make the antibody.
December 17, 20204 yr comment_81359 2 hours ago, John C. Staley said: Thankfully I'm R1R2!!!! 59 minutes ago, David Saikin said: Me too John See....you've already got the makings of a club. You can celebrate your CEce heterozygosity together.
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