Hi,
My Blood Bank is currently looking to perform autoadsorptions for our patients with warm autoantibodies, as we get quite a few and spend a lot of money sending them out to the reference lab. We recently purchased Immucor’s W.A.R.M. reagent, and we are attempting to validate it. We are comparing our results to what the reference lab reports out to us, but we are running into discrepancies. The last 2 patients that we sent to them, were reported to us as warm reactive autoantibodies present with no underlying allo antibodies. However when we performed absorption’s to compare, our panels showed what looked like antibodies with specificities, after the autoantibody was adsorbed out (one fit a little e pattern, and one looked like an anti-D). Since our results do not correlate with the reference lab results, I called them to find out what they treat their cells with. They said they make a home brew of papain and DTT together. The immucor W.A.R.M. that we used, according to the package insert says that it is lypholized papain and DTT , which is the same. I don’t understand why we would get such discrepant results? If anyone out there has some answers I would totally appreciate it!! How can we move forward with our validation if our results never match? Please help!! 🙂