kjaggers1 Posted July 23, 2020 Share Posted July 23, 2020 Why is bovine albumin used as a potientior in a lui freeze elution. Most procedures I have read does not require it. Thanks Link to comment Share on other sites More sharing options...
exlimey Posted July 23, 2020 Share Posted July 23, 2020 24 minutes ago, kjaggers1 said: Why is bovine albumin used as a potientior in a lui freeze elution. Most procedures I have read does not require it. Thanks Do you mean used to MAKE the eluate or it being added to the test system ? Link to comment Share on other sites More sharing options...
Baby Banker Posted July 24, 2020 Share Posted July 24, 2020 21 hours ago, kjaggers1 said: Why is bovine albumin used as a potientior in a lui freeze elution. Most procedures I have read does not require it. Thanks It has been eons since I did one of these, but I don't remember using albumin. I looked at a couple of procedures online, and it was not listed as a reagent. Link to comment Share on other sites More sharing options...
MAGNUM Posted July 24, 2020 Share Posted July 24, 2020 I have never added albumin to my Lui Freezes. BldBnker 1 Link to comment Share on other sites More sharing options...
David Saikin Posted July 24, 2020 Share Posted July 24, 2020 I've never added albumin either. BldBnker 1 Link to comment Share on other sites More sharing options...
AMcCord Posted July 27, 2020 Share Posted July 27, 2020 I've got an OLD OLD procedure that adds 3 drops of 30% albumin to 0.5 mL packed red cells prior to freezing. The reference is the 10th edition of the Technical Manual. The procedure is so old and so bare bones that there isn't any other information included - just the step by step. Looks really odd compared to current procedures. Link to comment Share on other sites More sharing options...
MAGNUM Posted July 27, 2020 Share Posted July 27, 2020 32 minutes ago, AMcCord said: I've got an OLD OLD procedure that adds 3 drops of 30% albumin to 0.5 mL packed red cells prior to freezing. The reference is the 10th edition of the Technical Manual. The procedure is so old and so bare bones that there isn't any other information included - just the step by step. Looks really odd compared to current procedures. All the albumin that I buy is 22% not 30%. Link to comment Share on other sites More sharing options...
tricore Posted July 27, 2020 Share Posted July 27, 2020 I don't remember the original paper (>40 years ago) by Anita Lui using albumin. Link to comment Share on other sites More sharing options...
AMcCord Posted July 27, 2020 Share Posted July 27, 2020 (edited) 7 hours ago, MAGNUM said: All the albumin that I buy is 22% not 30%. 30% albumin hasn't been available for years - I think we saw it last in the early 90s. I don't think I've ever used the Lui Freeze Thaw on a patient. I keep a copy of the ancient procedure around for students. Edited July 27, 2020 by AMcCord Link to comment Share on other sites More sharing options...
Baby Banker Posted July 28, 2020 Share Posted July 28, 2020 14 hours ago, AMcCord said: 30% albumin hasn't been available for years - I think we saw it last in the early 90s. I don't think I've ever used the Lui Freeze Thaw on a patient. I keep a copy of the ancient procedure around for students. The Lui Freeze Thaw method is good for demonstrating anti-A, anti-B, and anti-A,B from small samples. We used it to confirm that an infant had ABO HDN. I'm not sure if we still have a procedure for it. I work primarily with the Blood Bank Computer system now. Link to comment Share on other sites More sharing options...
AMcCord Posted July 28, 2020 Share Posted July 28, 2020 20 minutes ago, Baby Banker said: The Lui Freeze Thaw method is good for demonstrating anti-A, anti-B, and anti-A,B from small samples. We used it to confirm that an infant had ABO HDN. I'm not sure if we still have a procedure for it. I work primarily with the Blood Bank Computer system now. We don't do elutions to confirm ABO HDN, which is why I don't think I've ever used the Lui for a patient test. If Mom is type O with a negative antibody screen and baby is A or B with positive DAT, it's presumptive for ABO HDN. If there was evidence indicating that something else was going on (significant anemia, elevated bili that seems atypical for ABO HDN, etc.) we would investigate further. It is possible to use Elu-Kit II on a small sample, though we usually have cord blood to work with, so that is the method we would use for that extended investigation to make sure that we aren't dealing with a non-ABO antibody. My students have mixed success with the Lui, but we do it anyway. Hands on with methods like that seems to really help them understand the written material they are given. David Saikin 1 Link to comment Share on other sites More sharing options...
mrmic Posted July 29, 2020 Share Posted July 29, 2020 I somewhat remember that early on with the LUI freeze elution there were some attempts to elute non-ABO antibody specificities. At that time some suggested an additional source of protein and/or a minimal "LISS" environment might help with detecting these other antibody specificities. I'm not sure it worked out too well and other elution methods were much better. We just used for ABO elutes. David Saikin and AMcCord 2 Link to comment Share on other sites More sharing options...
Baby Banker Posted August 13, 2020 Share Posted August 13, 2020 On 7/28/2020 at 6:54 AM, Baby Banker said: The Lui Freeze Thaw method is good for demonstrating anti-A, anti-B, and anti-A,B from small samples. We used it to confirm that an infant had ABO HDN. I'm not sure if we still have a procedure for it. I work primarily with the Blood Bank Computer system now. I checked and we do not have a procedure for the LUI Freeze-Thaw technique any longer. Link to comment Share on other sites More sharing options...
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