I actually pulled the package insert for our panoscreen cells from Immucor, and on page 2 the insert actually says add plasma, then cells and mix. The the next line says add potentiator, the step to do the immediate spin is no longer present. I would never have noticed this if not for this blog. There is a note that if desired the immediate spin can be performed. I do believe that I will be having a discussion with my Director and Medical Director so that I can change the procedure because if it is not needed (which in my humble opinion it is not necessary) then we need to get rid of the steps.
scott