Reputation Activity
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Yanxia reacted to Malcolm Needs in Maternal alloantibody, not detected in baby - how long for antigen negative unitsArndt PA, Garratty G, Marfoe RA, Zeger GD. An acute haemolytic transfusion reaction caused by an anti-P1 that reacted at 37 degrees C. Transfusion 1998; 38(4): 373-377. DOI: 10.1046/j.1537-2995.1998.38498257376.x.
Smith D, Aye T, Er LS, Nester T, Delaney M. Acute hemolytic transfusion reaction due to anti-P1: a case report and review of institutional experience. Transfus Med Hemother 2019; 46: 381-384. Published online as DOI: 10.1159/000490897.
Irani MS, Figueroa D, Savage G. Acute hemolytic transfusion reaction due to anti-Leb. Transfusion 2015; 55: 2486-2488. DOI: 10.1111/trf.13178.
Delk AA, Gammon RR, Alvarez H, Benitez N, Bright F, A hemolytic transfusion reaction caused by an unexpected Leb antibody. Laboratory Medicine 2021; 52: 303-306. DOI: 10.1093/labmed/lmaa070.
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Yanxia reacted to Malcolm Needs in Maternal alloantibody, not detected in baby - how long for antigen negative unitsI'm sorry Neil, but Geoff Daniels quotes some HTR's caused by anti-N reacting at 37oC, and one case of mild HDFN in a M+ N+ baby, where the mother was M+ N-, S-, s- Uvar, in the third edition of his book, Human Blood Groups.
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Yanxia reacted to Arno in Maternal alloantibody, not detected in baby - how long for antigen negative unitsFor acquired maternal IgG antibodies (which may also be transferred postnatally through breast milk), assessing the antibody specificity (AbS) in the newborn, as previously mentioned, appears to be a reasonable approach. In addition, the Direct Antiglobulin Test (DAT) remains key, and performing an elution is important (even if the DAT result is negative). In your case, the negative DAT suggests that either the anti-N antibody did not cross the placenta, possibly due to being a naturally occurring IgM, and/or the baby is N-negative.
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Yanxia reacted to Malcolm Needs in Maternal alloantibody, not detected in baby - how long for antigen negative unitsIn that case, I would consider a genotype, as getting hold of M+ N-, S-s-U- fresh units is not going to be easy. That having been said, as you say yourself, anti-N is rarely clinically significant and, if it is not detectable in either the maternal circulation, or in the baby's circulation, I wouldn't worry too much about giving M+, N-, S-s-U- blood. BEAR IN MIND THOUGH, THIS WILL BE A CLINICAL DECISION, AND I AM NOT, AND NEVER HAVE BEEN, MEDICALLY QUALIFIED.
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Yanxia reacted to Malcolm Needs in Maternal alloantibody, not detected in baby - how long for antigen negative unitsThe reason I said this (and I admit that I am being more than a little "Reference Laboratory Pedantic here) is because a very good friend of mine (Edmond Lee, who used to work at the NHSBT-North London Centre at Colindale, with such luminaries as Prof Dame Marcela Contreras, Dr Mahes de Silva and Martin Redman, amongst others, who described a case where the bay of a woman with an extremely strong anti-K,, where the baby's foetal K antigens were blocked by the maternal anti-K, and so tested as negative (Lee E, Redman M, Owen I. Blocking of fetal K antigens on RBC by maternal anti-K. Transfus Med 2009; 19(3):139-40. doi: 10.1111/j.1365-3148.2009.00917.x. Later, he reported the same sort of thing with a maternal anti-Fy(a) (Lee E, Cantwell C, Muyibi KO, Modasia R, Rowley M, New H. Blocking phenomenon occurs with murine monoclonal antibodies (anti-Fya) in a neonate with a positive direct antiglobulin test due to maternal anti-Fy(a). Blood Transfus 2015; 13: 672-674. doi: 10.2450/2015.0232-14.
Obviously, in both these cases, the maternal antibody was easily detectable, so not the same as the case being described by BullDawgPath, and, in both cases, the baby's DAT was positive, BUT, in both cases, antigen negative blood was required by the baby.
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Yanxia reacted to Malcolm Needs in Maternal alloantibody, not detected in baby - how long for antigen negative unitsIt is incredibly rare for anti-N to be an alloantibody, unless the individual is M+N-, and also S-s-U-. This is because the amino acids that characterise the N antigen on the Glycophorin A molecule (leucine, serine, threonine, threonine, glutamic acid) are identical to the amino acids that characterise the 'N' antigen on the Glycophorin B molecule.
Is the lady of Black ethnicity by any chance? If not, to be N Negative AND 'N' Negative would be almost unique.
This suggests to me that the anti-N reported to be in the maternal circulation by the other hospital may well have been an auto-antibody, and would almost certainly be sub-clinical in its significance. In such a case, I would not bother with performing genotyping of the baby's N type. However, as far as Rh, K, etc, I would certainly suggest that antigen negative blood is given to the baby, and I certainly WOULD perform foetal genotyping (see my answer to Cliff above).
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Yanxia reacted to Malcolm Needs in Maternal alloantibody, not detected in baby - how long for antigen negative unitsAll great questions, but I would also ask, what is the baby's Hb/Hct requiring a transfusion, and why not test the baby's DNA for the gene encoding the antigen cognate to the maternal antibody?
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Yanxia reacted to John C. Staley in Maternal alloantibody, not detected in baby - how long for antigen negative unitsCouple of questions for clarification. What is the specificity of the known Alloantibody? "Baby is born and our testing shows negative antibody screen." Was this AB screen done on mom or baby? If on the baby, was a current ab screen performed on mom and if so what was the results? Was a DAT performed on the baby? If so, what was the result? If not, why not? Thanks
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Yanxia reacted to jtemple in Gel vs tube for DARA patientsWhat? All this time I have been using the wrong stuff! Ha! SPELL CHECK IS NOT YOUR FRIEND! 🤣🤣🤣🤣
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Yanxia reacted to Malcolm Needs in Gel vs tube for DARA patientsUm, sorry Jason, but I think you mean Dithiothreitol (DTT), rather than Dichlorodiphenyltrichloroethane (DDT)!!!!!!!
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Yanxia reacted to Neil Blumberg in Plasma transfusionsAlso, were any of the transfused units antigen positive? This is the quickest way to get a negative indirect antiglobulin test ;).
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Yanxia reacted to John C. Staley in Plasma transfusionsHow many units of uncrossmatched blood did they receive? How active were they bleeding? How much later did the other hospital preform their own T&S? Inquiring minds want to know!!! 😉
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Yanxia reacted to Malcolm Needs in Plasma transfusionsI agree Darin, it is almost certainly a dilutional effect, BUT, it could also be the effect of a soluble antigen (obviously not within the Duffy Blood Group System). If the antibody had a specificity within, for example, the Lewis Blood Group System, or the Chido/Rodgers Blood Group System, the antigen in the plasma could well adsorb out the circulating antibody. That having been said, this explanation is FAR less likely than your suggestion of the dilutional effect.
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Yanxia reacted to jojo808 in Need AdviceMy greatest apology for leaving you all hanging. We've been so incredibly busy and short-staffed that I could not even think about anything else but trying to finish up my daily duties. Anyway, seems that the patient also had an impella device that had to be "adjusted" and I believe that corrected the hemolysis. I'm only reading the responses today (2 weeks later)☹️so hats off to you all who thought mechanical causes. I would have not thought that the device would be that far-off to cause the gross hemolysis we saw. We do see slight hemolysis with impella devices but not like this one. I guess never say never. Thank you all for your responses.
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We have seen this phenomenon from time to time, albeit rarely. We use R1wR1 red cells with all of our antibody identifications, purely because it is always cell 1 in the panel! We also, however, use two R1R1 panel cells and an r'r panel cell.
I'm not too sure why we get the odd sample that reacts like that, because the C antigen on the R1wR1 panel cell is not that much weaker than the C antigen on the R1R1 panel cells, and certainly no weaker than that on the r'r panel cells.
Remember that the "w" of "Cw" stands for "Willis", and not "weak", as it was named after the donor who caused the immunisation to the antigen in the first example of anti-Cw described (something I forgot a few years back in an article I wrote, much to my embarrassment, if that is any solace to you) and that RH8 (RHCw) is allelic to both RH9 (RHCx) and RH51 (MAR), and not to either RH2 (RHC) or RH4 (RHc). Therefore, any weakening must be due to steric hinderance, or something similar.
:confuse::confuse:
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Yanxia reacted to Bet'naSBB in Eliminating Immediate spin for cold agglutininsAt our facility, we have to do a cold adsorption IF the cold auto interferes with the reverse and/or ISXM. Since testing with "neat" plasma is our standard of practice, we would still report the XM as incompatible and send it out with a release stating the incompatibility was due to an autoantibody. We are not "allowed" to prewarm away IS reactions. 🥶 IF we prewarm - it's only if the cold has a high thermal amplitude and causes interference after 37deg incubation / antiglobulin phase...........but that's just us.........
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Yanxia reacted to DLabGirl in Suspected Anti-D + Anti-C vs. Anti-G: Separation/Differentiating Difficulty — Has Anyone Seen Similar Results? Case Comparisons? Thoughts...Happy Friday everyone!
First off, Thank you Malcolm 😊 for that excellent PPT lecture! 👌 With your permission, I'd love to share it with my students; it's exactly the kind of content that helps bring these complex concepts to life (and mildly melt brains in the best way possible).
Speaking of melted brains… let's talk Anti-G differentiation, shall we? While I agree that differentiation isn’t necessary for transfusion purposes because in cases where anti-G is suspected, the recommendation is the same (D and C antigen-negative PRBCs); in the US Reference Lab world, it's a whole different dance. Differentiating anti-D + anti-C from anti-G is essential in alloimmunized patients with anti-D + anti-C for Rh Immune Globulin (RhIg) prophylaxis administration indication. Medical teams want to know if a patient is a candidate for RhIG, and if it's still indicated, especially to avoid future medical and legal complications. And so begins the beautiful (read: painstaking) double adsorption and elution process. 😫
The presence/development of a real anti-D indicates the patient does not require RhIg administration, whereas the presence of anti-G indicates the need for RhIg prophylaxis. That way, RhIG is avoided in patients with a real anti-D (although clinical correlation is recommended to guide every decision).
In this case, we saw what looked/reacted like anti-D and anti-C (1). Adsorption with R2R2 cells showed anti-C in the adsorbed serum (2) and anti-D and/or G in the adsorbing R2R2 cell eluate (3). Usually, we would stop and call anti-C and anti-D, if we have no reactions on C Ag Pos cells, but since suspected anti-G was in the mix, we routinely differentiate/separate it. The anti-D was confirmed in (4) by adsorbing the eluate containing the suspected (anti-D + anti-G) with an r'r unit. So far, so good. However, when we tried to separate the anti-G by elution of the adsorbing r′r cells, expecting a reaction on D and C antigen-positive cells (5), we got a negative result (Cue dramatic music).
I know, I know...at this point you’re probably wondering if we’re still in the Blood Bank or if we’ve accidentally wandered into theoretical physics. Trust me, my brain also wobbles when explaining G differentiation. It's the kind of thing that makes you rethink your career choices... for about 5 minutes... and then roll up your sleeves and start prepping another adsorption. 😅
A couple of housekeeping: We ficin treat our adsorbing cells, and sometimes use PEG in adsorption for efficiency. After briefly considering a career in something less chaotic, we retraced our steps. No PEG this time, 60-minute incubation, followed up with a DAT after each adsorption to check for antibody coating... and voilà: finally got the positive reaction we were looking for. The patient has anti-D, anti-C, and anti-G.
I can only think of a possible weak DAT strength pending elution, or PEG interference when used in adsorption. As always, this is Blood Bank, we know a million things can go wrong, and often do. But in the end, science (and a lot of perseverance) wins.
Thanks again to everyone for your input, patience, your brains, and your sense of humor. And again, Malcolm, thanks for your lecture, teaching, dedication and inspiration!
I wish everyone a calm weekend. May your panels be clear, your DATs negative, and your eluates informative. 😉
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Yanxia got a reaction from John C. Staley in Forward and reverse blood grouping in a donor centreIn our lab, we do 30 patients ABO typing daily in average. In those tests we will find out forward and reverse typing mismatch at least once daily. Maybe because we tested patients' sample, the incidence is higher than donors', but just as Malcolm said it is definitely necessary to do forward and reverse typing and make sure they are matching.
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Yanxia reacted to Malcolm Needs in Suspected Anti-D + Anti-C vs. Anti-G: Separation/Differentiating Difficulty — Has Anyone Seen Similar Results? Case Comparisons? Thoughts...In the UK, we would test serum/plasma samples from pregnant patients to see if there was an anti-C + Anti-G, or an anti-G on its own, but if the tests showed an anti-D+C, we didn't go any further to see if there was an anti-G there as well. I mean, what for? What difference does it make?
I attach a PowerPoint lecture on the subject I wrote some years ago, but I think it is still pertinent.
The G Antigen and Anti G.pptx
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Yanxia got a reaction from Ensis01 in Forward and reverse blood grouping in a donor centreIn our lab, we do 30 patients ABO typing daily in average. In those tests we will find out forward and reverse typing mismatch at least once daily. Maybe because we tested patients' sample, the incidence is higher than donors', but just as Malcolm said it is definitely necessary to do forward and reverse typing and make sure they are matching.
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Yanxia got a reaction from Malcolm Needs in Forward and reverse blood grouping in a donor centreIn our lab, we do 30 patients ABO typing daily in average. In those tests we will find out forward and reverse typing mismatch at least once daily. Maybe because we tested patients' sample, the incidence is higher than donors', but just as Malcolm said it is definitely necessary to do forward and reverse typing and make sure they are matching.
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Yanxia reacted to Malcolm Needs in Source of advice on transfusing patients with mismatched stem cell/bone marrow transplants?Sorry Neil, but I have to point out that this is not completely accurate. Any red cell antigens that are adsorbed onto the red cell surface, rather than being an integral part of the red cell membrane remain the type of the patient, rather than the donor. This is true of the Lewis phenotype (for instance, if the recipient was Le[a+b-], and the donor was Le[a-b+], after the transplant, the red cells will group as Le[a+b-], and not as Le[a-b+]}. This is also true of antigens within the Chido/Rodgers Blood Group System, and certain others.
If the recipient is a Secretor, they will continue to secrete ABO substance of the original ABO type, which, of course, will also be adsorbed onto the red cell surface (as well as being in the plasma, leading to the phenomenon of "accommodation", and this is why most recipients stay with a reverse group of "AB" after an ABO mis-matched stem cell/bone marrow transplant.
SORRY TO BE A PEDANT, PARTICULARLY AS I AGREE WITH EVERYTHING ELSE YOU HAVE WRITTEN!
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Yanxia reacted to Malcolm Needs in +s in Ortho panelThe +s stands for strongly expressed.
The expression of the P1 antigen varies considerably from person to person, but the reaction strength with anti-P1 is an inherited trait (i.e. the strength of the expression on the red cell surface).
"I apologize for this dumb question." BBnoob69, NO QUESTION IS A DUMB QUESTION, IF YOU DO NOT KNOW THE ANSWER. If you don't know the answer, the dumb thing is to not ask the question in the first place. NEVER be afraid to ask a question on here,
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Yanxia reacted to Malcolm Needs in Cw reactivityHi Rich,
Yes you can, and, don't forget, under BSH Guidelines, you do not have to give blood that has been tested for the Cw antigen, if the unit is compatible by IAT with the patient's plasma/serum. It is one of the few Rh antigens that can be given under these circumstances.
Be aware though, that I answer this in the knowledge that you are working in the Isle of Man (i.e. the UK). This may not apply in other parts of the world (particularly Lithuania and Finland).
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Yanxia reacted to Malcolm Needs in Cw reactivityALL Rh antibodies react with red cells treated with proteolytic enzymes, such as ficin, papain, trypsin and alpha-chymotrypsin (well, red cells that are expressing the cognate antigen, anyway), BUT, be careful because most monoclonal grouping reagents, including monoclonal anti-Cw, will often say to be used by either direct agglutination or by IAT, BUT NOT to be used with enzyme-treated red cells, because they can cause false positives.
Most of what I have written above can be found in Reid ME, Lomas-Francis C, Olsson ML. The Blood Group Antigen FactsBook. 3rd edn, 2012. Academic Press. ISBN: 978-0-12-415849-8. The rest can be found in the manufacturer's insert, if the reagent is commercial.
Hope that helps, but feel free to get back if it doesn't.