yan xia

it is a long post to me the first one, i often see B antigens are weaker than A antigens on our newborns, but ont as weak as 1+, i think it maybe an ABsubgroup. the second one,"Lutheran antibodies have not been implicated in immediate haemolytic transfusion reactions, although they may have been responsible for mild delayed reactions and post-transfusion jaundice."I think the symptom after transfusion fit it. Geoff Daniel, Human bloog groups,second edition, 279,230

Sir, do you mean the anti-A1 in the A2 individual will destruct the transfused A1 cells where the circulation temperature is lower than 37oc, even the anti-A1 has no reaction at 37oc

the anti-A1 idoes rarely react at 37 degree Celsius, but the anti-A or/and anti-B, anti-AB do.

just my personal opinion, when the antigens and antibodies reaction, there is a formula, in this case, the antigens( the binding antibodies on the cells) is fewer,( we can see this from the reaction strength w+), so add more antigens will give stronger reaction. of course it is in a range.

first of all, i have not done this test before.my opinion is just from my understanding of the threats i read before, just to express myself, myabe and most likely it is not right. the auto control is the reaction of patient's cells with its own plasma, the DTT treatment is for the screening cells, if i understand it rightly. so the DTT does not do anything to the autocells.

i guess the difference is because the second time's cells is more than the first time( two drops vs. one)

we will not transfuse in this situation

i am sorry if my understanding is wrong. Do you mean if the salin panel result is neg, you will not do adsorption test even the enzyme and PEG/LISS panel results is pos? I think it is not safe, because most allo -antibodies are IgG, they are non reactive in saline.

and from my daily work, i find we can tell from the proportion of cells, if transfused is less, then on the mix field result, the large part is the patient's own cells

there is a method which using a microtube( sorry, i am not sure how to call it), fill the tube with blood, then centrifuge it, the new generated red cellls are lighter so they are on the upper layer, they are the patients' own cells, the transfused cells are heavier, so they are on the bottom

It seems a weak anti-D which is sometimes too weak to show up on enzyme technique. Maybe the former several pregnancies immune the lady.Maybe knowing the kids D type will help.

i cannot remember we do in-dated panel cells QC in the reference lab use antiserum . Just as Debbiel mentioned the panel cells must be visually qualified. we use them to do antibodies identification, then get the result, use reagent antiserum to confirm the antigen is absence on the auto cells if DAT is neg( if we have those reagent), then use two antigen pos cells and two antigen neg cells to confirm the antibodies again. the panel cells express a lot of antigens , some of them are rare and it is hard to get the specific antiserum, i think it is hard to QC those antigens during the life span of the panel.

The patient is AB, I don't think the transfused Rbcs are the problem. Maybe because the A platelets with high titer of anti-B or the patient's blood volume is smaller than normal adult. I think do an elution is good to prove what is on the red cells, there are lots of ABO HDFN has neg DAT, but pos elution.

The patient is AB, I don't think the transfused Rbcs are the problem. Maybe because the A platelets with high titer of anti-B or the patient's blood volume is smaller than normal adult. I think do an elution is good to prove what is on the red cells, there are lots of ABO HDFN has neg DAT, but pos elution.