yan xia

There are a lot of brilliant explanations, I learned so much from here. My guess about the pos DAT is that if the DAT(IgM) is positive, then it may cause false positive result in the D testing. This is why when we test a sample which is AB Dpos, we will run a neg control for the forward typing.

How about the DAT of this donor?

What does EBT mean? Thanks a lot.

Is there any chance of the anti-B in the donor packed cells caused the heamolysis? Because the free antibodies attached to the patient's cells, complements actived and cells hemolysis, so there is no free anti-B detected after transfusion.

or there are unexpected antibody/antibodies in the patient's plasma.

That was new to me, is it because the auto-cells are antigen-depressed by the autoantibodies or some other reasons?

The DAT and Auto control negative, do we need to do auto-adsorption?

I am not sure about the difference between B(A) and AsubB, I always thought we define B(A) based on genotype not phenotype, and the AsubB maybe the genetic product of B(A).

I guess there are two possibilities: 1.This patient is AsubB, the A antigen cannot be tested by some anti-A reagent. 2.This patient maybe has some antigen rather than A which is crossreacted with the component of the reacted reagent. solution are 1.How about test the patients' red cells against some B type human serum, please make sure that there are O cells as negtive control. The monoclonal reagents are not as complete as the human source polyclonal antibodies. 2.And to test the saliva for ABH substance in it( if the patient is a secretor). And do genotyping, just s

I totally agree with all those briliant ideas, and there are some A subgroup have anti-A and anti-A1, such as Ax and Ael .

I have a little confusion, why the previous auto tests are all neg, and the DAT is pos? The elution result is 4+, so I guess this DAT is IgG pos, and the anti-IgG in the prewarm test, the auto is neg. My not good English, I hope I understand and express it rightSorry for the interruption.

Seems as warm autoantibodies which have a loose reaction temperature span, so they can interfere with the reverse typing. Because they are not cold auto, so cold autoadsorption does not work, and in the strict prewarm test, they still react.

We use Ortho Biovue gel card, and I don't think rouleaux interfere much on the gel result, some MM patients have rouleux on tube, but gel results are good. In my experience, cold antibodies can do. Just personal opinion.

Malcolm, may I ask what is Gm and Km, and why typing those can tell us if the one is passive or allo-anti-D? Thank you .