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yan xia

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yan xia last won the day on October 10 2017

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About yan xia

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    Seasoned poster
  • Birthday 08/25/1982

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  • Gender
    Female
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    reading, travel,talk with a good friend et al.
  • Location
    China
  • Occupation
    bloodtransfusion department in hospital
  • Real Name
    Yanxia Wang

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  1. Can leuko-reduce prevent GVHD

    I guess irradiation can inactivate the lymphocytes, but not deprave the antigens it takes, so it can not replace leukoreduction.
  2. e and C titer

    Maybe you can adsorb the anti-e with ccee cells, then to see if there are still reaction with Ce cells, then you can figure out if there are anti-C here.
  3. ABO incompatibility

    Thank you very much for the explanation. Just a little confusion how do the Ca++, Mg++ and Mn++ ions infect the agglutination? does it because the complements can enhance agglutination or because the complements caused haemolysis is a sign of ABO mis-match?
  4. ABO incompatibility

    Would you explain this please? Thanks
  5. wAIHA with IgM and C3c/C3d coating

    Thanks Malcolm for your wonderful explanation. what is two-stage indirect antiglobulin technique, does it mean we add extra complements from fresh serum? I remember some method like that, but I am not sure about the name.
  6. I remember when I was as an intern in Micro department, there were a lot of specimens, some sent there to do direct organism tests using the dyeing technique under a microscop, which was quick but not so sensitive. So most of the specimens needed to be cultured for 24 hours to see what will happen. I will report positive in this case.
  7. I think this phenomenon is because the B antigens are not well developed on new born baby. BTW, I prefer to use 3+mf to describe it The reason I don't use 4+mf because 4+ agglutination is a kind of solid agglutination, without free cells .maybe I was wrong, just personal opinion.
  8. M antigen and antibody

    Yes, we use this kind of method to determine if there is ABO HDN present, putting the elution with reagent A,B,O cells respectively in the refrigerator( 4 degree C) overnight, then gently tip the tubes to see the agglutination. The O cells is as a negtive control, in case there are other cold antibodies interfere with the test.
  9. An Enigma (for me)

    Sorry, it was my mistake. I am very sorry.
  10. An Enigma (for me)

    Maybe it is some kind of combined antibody, such as anti-Alea?
  11. An Enigma (for me)

    The reagent A1 cells we used is a combination of three donor cells which produced by the factory.If the reaction with A1 cells is due to low antigens, maybe we can see mixed field reaction. I totally agree with your choice of choosing another A1 cells to do the test.
  12. Screen pos, xmatch neg?

    This is a complicated case. Are the screening cells reacting very good ( I mean does it have neg reaction) with other patients' sample?
  13. 2 Mysteries

    it is a long post to me the first one, i often see B antigens are weaker than A antigens on our newborns, but ont as weak as 1+, i think it maybe an ABsubgroup. the second one,"Lutheran antibodies have not been implicated in immediate haemolytic transfusion reactions, although they may have been responsible for mild delayed reactions and post-transfusion jaundice."I think the symptom after transfusion fit it. Geoff Daniel, Human bloog groups,second edition, 279,230
  14. Sir, do you mean the anti-A1 in the A2 individual will destruct the transfused A1 cells where the circulation temperature is lower than 37oc, even the anti-A1 has no reaction at 37oc
  15. the anti-A1 idoes rarely react at 37 degree Celsius, but the anti-A or/and anti-B, anti-AB do.
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