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yan xia

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yan xia last won the day on October 10

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About yan xia

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    Seasoned poster
  • Birthday 08/25/1982

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    reading, travel,talk with a good friend et al.
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    bloodtransfusion department in hospital
  • Real Name
    Yanxia Wang

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  1. wAIHA with IgM and C3c/C3d coating

    Thanks Malcolm for your wonderful explanation. what is two-stage indirect antiglobulin technique, does it mean we add extra complements from fresh serum? I remember some method like that, but I am not sure about the name.
  2. I remember when I was as an intern in Micro department, there were a lot of specimens, some sent there to do direct organism tests using the dyeing technique under a microscop, which was quick but not so sensitive. So most of the specimens needed to be cultured for 24 hours to see what will happen. I will report positive in this case.
  3. I think this phenomenon is because the B antigens are not well developed on new born baby. BTW, I prefer to use 3+mf to describe it The reason I don't use 4+mf because 4+ agglutination is a kind of solid agglutination, without free cells .maybe I was wrong, just personal opinion.
  4. M antigen and antibody

    Yes, we use this kind of method to determine if there is ABO HDN present, putting the elution with reagent A,B,O cells respectively in the refrigerator( 4 degree C) overnight, then gently tip the tubes to see the agglutination. The O cells is as a negtive control, in case there are other cold antibodies interfere with the test.
  5. An Enigma (for me)

    Sorry, it was my mistake. I am very sorry.
  6. An Enigma (for me)

    Maybe it is some kind of combined antibody, such as anti-Alea?
  7. An Enigma (for me)

    The reagent A1 cells we used is a combination of three donor cells which produced by the factory.If the reaction with A1 cells is due to low antigens, maybe we can see mixed field reaction. I totally agree with your choice of choosing another A1 cells to do the test.
  8. Screen pos, xmatch neg?

    This is a complicated case. Are the screening cells reacting very good ( I mean does it have neg reaction) with other patients' sample?
  9. 2 Mysteries

    it is a long post to me the first one, i often see B antigens are weaker than A antigens on our newborns, but ont as weak as 1+, i think it maybe an ABsubgroup. the second one,"Lutheran antibodies have not been implicated in immediate haemolytic transfusion reactions, although they may have been responsible for mild delayed reactions and post-transfusion jaundice."I think the symptom after transfusion fit it. Geoff Daniel, Human bloog groups,second edition, 279,230
  10. Sir, do you mean the anti-A1 in the A2 individual will destruct the transfused A1 cells where the circulation temperature is lower than 37oc, even the anti-A1 has no reaction at 37oc
  11. the anti-A1 idoes rarely react at 37 degree Celsius, but the anti-A or/and anti-B, anti-AB do.
  12. just my personal opinion, when the antigens and antibodies reaction, there is a formula, in this case, the antigens( the binding antibodies on the cells) is fewer,( we can see this from the reaction strength w+), so add more antigens will give stronger reaction. of course it is in a range.
  13. Testing using DTT-treated cells

    first of all, i have not done this test before.my opinion is just from my understanding of the threats i read before, just to express myself, myabe and most likely it is not right. the auto control is the reaction of patient's cells with its own plasma, the DTT treatment is for the screening cells, if i understand it rightly. so the DTT does not do anything to the autocells.
  14. i guess the difference is because the second time's cells is more than the first time( two drops vs. one)
  15. febrile transfusion reaction

    we will not transfuse in this situation