Mabel Adams 1,044 Posted March 21, 2016 Has anyone tried running Ortho's 0.8% panel cells in Grifols IgG cards? Share this post Link to post Share on other sites
goodchild 528 Posted March 21, 2016 No, but I'm already contemplating purchase of Grifols' antibody panel. We currently purchase Immucor 16 & 20 cell 3% panels and dilute to 0.8% for selected cells in addition to Resolve A and B 0.8%. Share this post Link to post Share on other sites
Auntie-D 363 Posted March 22, 2016 Don't griffols use a totally different cell:plasma ratio to Ortho though? I thought Ortho were 50ul:40ul and griffols were 50ul:25ul Share this post Link to post Share on other sites
David Saikin 1,280 Posted March 22, 2016 Grifols is the same as Ortho (50uL/25uL - at least in the usa), Biorad uses/will use 0.6% cell suspensions but I don't know about the cell/plasma ratio. Share this post Link to post Share on other sites
Auntie-D 363 Posted March 22, 2016 Ah... Ortho in the UK uses different ratios. Share this post Link to post Share on other sites
noelrbrown 27 Posted March 22, 2016 Be careful as the diluents used to suspend the cells will be different. For gel card assays you need a Low Ionic strength diluent. Diluents often contain potentiators and will have been validated for use in the appropriate card system. I am not saying they won't work and they probably will however you should perform validation to ensure you don't miss a significant antibody. 3 David Saikin, Malcolm Needs and JustaKIDD reacted to this Share this post Link to post Share on other sites
