Jives Posted June 2, 2014 Share Posted June 2, 2014 Does anyone out there run their ELU-KIT and/or gentle heat elutions with solid phase testing (ECHO)?If so, do you have a validation plan?? thanks, jane Link to comment Share on other sites More sharing options...
AMcCord Posted June 3, 2014 Share Posted June 3, 2014 I'm intrigued by the idea, but I've never tried it. Link to comment Share on other sites More sharing options...
David Saikin Posted June 3, 2014 Share Posted June 3, 2014 You should develop your own validation plan: 1. what you are going to validate. 2. Details of what you are going to do to perform you validation. 3. The results you expect. 4. The results you obtain. 5. Evaluation: if you get the results you expect you are ready to go. If not, you decide whether to alter your procedure and try again or decide that you cannot validate your idea. I would say you are going to compare elution studies between your instrument and your current methodology (tubes?). Whether you make up samples or use pt samples is up to you; how many you run is up to you. Link to comment Share on other sites More sharing options...
Jives Posted June 4, 2014 Author Share Posted June 4, 2014 i've been running them in duplicate for years and have just been lazy about writing a validation plan.....the most interesting one i ran was an ECHO only anti-Jka with a neg DAT.....the ELU-KIT eluate was negative in tubes, but 3+ positive on ECHO in all three screening cells!!! Link to comment Share on other sites More sharing options...
AMcCord Posted June 4, 2014 Share Posted June 4, 2014 Jives... the anti-Jka is interesting. In general, how do your results compare between tube and the Echo - mostly the same results? Are the reactions stronger with Echo? Link to comment Share on other sites More sharing options...
Eagle Eye Posted June 5, 2014 Share Posted June 5, 2014 I am almost certain that ECHO will be more sensitive then Tube?Same is the case for Gel.we some time run eluate in gel when we suspect an antibody. Multiple occasion Tube was negative and gel picked up true antibody. Link to comment Share on other sites More sharing options...
Jives Posted June 5, 2014 Author Share Posted June 5, 2014 Of the approx. 25 i have, only 2 are non-concordant and those are pos with ECHO and neg with tube (the prev ECHO only Jka being one of them) and yes, they are always stronger with ECHO than tube. AMcCord 1 Link to comment Share on other sites More sharing options...
David Saikin Posted June 6, 2014 Share Posted June 6, 2014 i've been running them in duplicate for years and have just been lazy about writing a validation plan.....the most interesting one i ran was an ECHO only anti-Jka with a neg DAT.....the ELU-KIT eluate was negative in tubes, but 3+ positive on ECHO in all three screening cells!!! Just curious why you were running an elution on a neg DAT? (not that I have not done the same thing) Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted June 6, 2014 Share Posted June 6, 2014 Sachs UJH, Roder L, Santoso S, Bein G. Does a negative direct antiglobulin test exclude warm autoimmune haemolytic anaemia? A prospective study of 504 cases. British Journal of Haematology 2006; 132: 651-661. In this, they not only talk about WAIHA, but also quite a bit about delayed serological transfusion reactions, and, most importantly, delayed haemolytic transfusion reactions that are DAT negative, but which show clinical symptoms. Link to comment Share on other sites More sharing options...
Jives Posted June 9, 2014 Author Share Posted June 9, 2014 i've been running them in duplicate for years and have just been lazy about writing a validation plan.....the most interesting one i ran was an ECHO only anti-Jka with a neg DAT.....the ELU-KIT eluate was negative in tubes, but 3+ positive on ECHO in all three screening cells!!! Just curious why you were running an elution on a neg DAT? (not that I have not done the same thing) i just have a feeling that the increased amount of anti-Jka's we see on ECHO might really be developing warm autos (or just extremely weak ones that only ECHO can detect) and i wanted to test my theory by doing an eluate on one of them Link to comment Share on other sites More sharing options...
David Saikin Posted June 10, 2014 Share Posted June 10, 2014 Malcolm I have read that article and performed some elutions on cells with a neg DAT. A lady at work was having transient fevers and a lowering hct, Her docs and the referral docs were not finding anything. We did an elution on her neg DAT and found a 2+ panagglutinin in gel. Very interesting but none of the docs (except) my Medical Director would take notice. Eventually she was put on steroids for some obscure disease of her epithelial cells. Her eluate became negative. Definitely interesting. Malcolm Needs and AMcCord 2 Link to comment Share on other sites More sharing options...
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