Complement Control Cells

[Jane]

Is everyone using the complement control cells:

TRM.40210 DAT Phase II

When performing an antiglobulin test with anti-C3 antiglobulin reagents, C3-coated red blood

cells are used as a control in all negative antiglobulin tests.

and where are you getting them from? I'm not happy with my current vendor (they don't come on a predictable standing order) and I just saw that Hemobioscience has them. Any experience with that vendor?

Thanks!

[David Saikin]

I use Immucor's product - significantly less expensive than HemoBioscience (due to purchasing programs). I receive them regularly with no problem. Using them in tube is arduous in that you really have to try to get a decent 1+ reaction. I use them in buffered gel with anti-C3b,-C3d - they are always 3-4+. I have heard that Quotient is working on a kit to allow you to make your own Complement + check cells.

[pbaker]

We use Immucor and have not had any problems with our standing order. It does help if you let the check cells sit a few minutes before you read them. They tend to be stronger.

[tupton]

We switched to Bioscience from Immucore because of price

[cthherbal ☆]

We do the same as David. Beautiful reactions in gel.

[AMcCord]

We use the Immucor cells. No shipping problems and we always see nice reactions in tube. (We do have a contract that gives us good pricing.)