Immediate Spin crossmatch using Buffered Gel card

[Walter]

Do you use the Buffered Gel card for immediate spin crossmatch [instead of tube]?

If so, what hospital/lab group are you from.

Doing research for our technical advisory team.

Thanks,

Walter

[Malcolm Needs ☆]

As we are a Reference Laboratory, any cross-match we perform involves an atypical alloantibody or an auto-antibody, and so we never perform an immediate spin cross-match.

Sorry Walter, but I don't suppose that my post is of any help to you or your technical advisory team, but I wish you well with your research.

[PAWHITTECAR]

We use tube IS crossmatched. We have had trouble with the buffered cards in the past supressing antibodies, particularly Kell so we do not use them.

[Likewine99]

Yes we use buffered gel cards on the ProVue for ISXM and have for about 6 years. Use tube ISXM for traumas and massive bleeders.

SSM Healthcare, St. Louis, MO, DePaul Health Center, 350 bed Level II trauma center.

[teskridge]

Yes, we use the buffer cards on our ProVues. McLeod Regional Medical Center in Florence, SC

[Mabel Adams]

Since you would be using these for detecting ABO incompatibility, would you be concerned about them not being able to detect anti-K?

[Malcolm Needs ☆]

Since you would be using these for detecting ABO incompatibility, would you be concerned about them not being able to detect anti-K?

Am I being particularly thick Mabel, or is that a play on words concerning McLeod Regional Medical Center???

[teskridge]

I must not be understanding the question...what has the buffer cards and performing the IS crossmatch in gel got to do with detecting Anti-Kell?

[PAWHITTECAR]

We have seen instances where the K antibody that we use as part of our QC for panels did not react as expected when using the buffered cards. We finally completely quit using buffered cards.

[Malcolm Needs ☆]

Ah, didn't read your original post properly Trish, in which case my reply to Mabel was totally rubbish. Sorry Mabel.

[PAWHITTECAR]

Oh we're all allowed to spout rubbish occasionally

[drwajiha]

immediate spin crossmatch is done in only in case of emergency when you do not have time to do a complete compatibility testing and the objective is to rule out ABO incompatibility. Practically a lot of time is saved doing it in tube instead of using gel card. Kindly anyone will explain the logic / scientific reasoning of using gel card under these circumstances. I would be much obliged.

[Mabel Adams]

In the US immediate spin crossmatch is not limited to emergencies. It could be used for any patient with a negative antibody screen and no history of significant antibodies. An antiglobulin crossmatch is required only if the above conditions are not met.

[Malcolm Needs ☆]

In the US immediate spin crossmatch is not limited to emergencies. It could be used for any patient with a negative antibody screen and no history of significant antibodies. An antiglobulin crossmatch is required only if the above conditions are not met.

Same in the UK.

[KarenJ]

We use tube for IS crossmatch. Lawrence Memorial Hospital Lawrence Ks

[David Saikin]

We use tube also (for ISXM).