JeanB Posted October 25, 2024 Posted October 25, 2024 Are there any guidelines available for having to require a QC performed when converting 3% RBC suspension to 0.8%? Even when the panels are in-date? If you are doing this at your facility, can you share your approach?
Bet'naSBB Posted October 25, 2024 Posted October 25, 2024 We use Ortho-Vision Max and manual gel techniques. During our validation for manual testing, we found that we sometimes missed reactivity in the IgG card when cells were diluted to 0.8% and incubated for only 15 minutes. The reactivity was then present when incubation was extended to the maximum allowable time for the IgG cards so that's what is now in our SOP. Yanxia 1
JeanB Posted October 26, 2024 Author Posted October 26, 2024 On 10/25/2024 at 9:47 AM, Bet'naSBB said: We use Ortho-Vision Max and manual gel techniques. During our validation for manual testing, we found that we sometimes missed reactivity in the IgG card when cells were diluted to 0.8% and incubated for only 15 minutes. The reactivity was then present when incubation was extended to the maximum allowable time for the IgG cards so that's what is now in our SOP. Does your facility run QC when diluting to 0.8%?
RRay Posted October 28, 2024 Posted October 28, 2024 I have the official conversion guide from Ortho and it doesn't recommend any specific QC apart from the general QC of the method it alludes to (the general MTS procedure manual). I mention visual inspection of cell button size in my SOP. Has always covered it in the past, but you know how it goes... Quick Reference Quide - Conversion.pdf SbbPerson and John C. Staley 2
Mabel Adams Posted October 29, 2024 Posted October 29, 2024 On 10/24/2024 at 8:34 PM, JeanB said: Are there any guidelines available for having to require a QC performed when converting 3% RBC suspension to 0.8%? Even when the panels are in-date? If you are doing this at your facility, can you share your approach? Aren't your crossmatch QC testing cells diluted in diluent 2 to 0.8%? That should cover QC of the dilution process. Also, we do tube QC on the 3% screen cells every day which should cover antigen integrity.
Bet'naSBB Posted October 30, 2024 Posted October 30, 2024 On 10/26/2024 at 1:04 PM, JeanB said: Does your facility run QC when diluting to 0.8%? We only run QC if we are diluting an expired cell. (we run QC on any expired cell - whether we dilute or not) We performed a validation before beginning and validated that the dilution worked if incubated for 40 minutes which is the longest allowable time to incubate per the IFU.
exlimey Posted October 31, 2024 Posted October 31, 2024 On 10/24/2024 at 11:34 PM, JeanB said: Are there any guidelines available for having to require a QC performed when converting 3% RBC suspension to 0.8%? Even when the panels are in-date? If you are doing this at your facility, can you share your approach? Are you diluting Ortho 3% cells down to 0.8%, or another manufacturer's ?
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