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Posted

Are there any guidelines available for having to require a QC performed when converting 3% RBC suspension to 0.8%? Even when the panels are in-date? 

If you are doing this at your facility, can you share your approach? 

 

Posted

We use Ortho-Vision Max and manual gel techniques.  During our validation for manual testing, we found that we sometimes missed reactivity in the IgG card when cells were diluted to 0.8% and incubated for only 15 minutes.  The reactivity was then present when incubation was extended to the maximum allowable time for the IgG cards so that's what is now in our SOP.

Posted
On 10/25/2024 at 9:47 AM, Bet'naSBB said:

We use Ortho-Vision Max and manual gel techniques.  During our validation for manual testing, we found that we sometimes missed reactivity in the IgG card when cells were diluted to 0.8% and incubated for only 15 minutes.  The reactivity was then present when incubation was extended to the maximum allowable time for the IgG cards so that's what is now in our SOP.

Does your facility run QC when diluting to 0.8%? 

Posted
On 10/24/2024 at 8:34 PM, JeanB said:

Are there any guidelines available for having to require a QC performed when converting 3% RBC suspension to 0.8%? Even when the panels are in-date? 

If you are doing this at your facility, can you share your approach? 

 

Aren't your crossmatch QC testing cells diluted in diluent 2 to 0.8%?  That should cover QC of the dilution process. Also, we do tube QC on the 3% screen cells every day which should cover antigen integrity.

Posted
On 10/26/2024 at 1:04 PM, JeanB said:

Does your facility run QC when diluting to 0.8%?

We only run QC if we are diluting an expired cell. (we run QC on any expired cell - whether we dilute or not)

We performed a validation before beginning and validated that the dilution worked if incubated for 40 minutes which is the longest allowable time to incubate per the IFU. 

Posted
On 10/24/2024 at 11:34 PM, JeanB said:

Are there any guidelines available for having to require a QC performed when converting 3% RBC suspension to 0.8%? Even when the panels are in-date? 

If you are doing this at your facility, can you share your approach? 

 

Are you diluting Ortho 3% cells down to 0.8%, or another manufacturer's ?

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