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dothandar last won the day on July 21

dothandar had the most liked content!

About dothandar

  • Rank
    Junior Member
  • Birthday 07/23/1982

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  • Gender
    Not Telling
  • Interests
    I am obsessed with actions going on red cell surface
  • Location
    Seattle, WA
  • Occupation
    IRL tech

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  1. dothandar

    Mycoplasma pneumoniae

    I love made up cases!!! Do we get more information in this case after we answer your questions? Anyhow, here are my thoughts. "Female patient unknown transfusion history" I would perform antigen typing for D,C,E,c,e,K,S,s,Fya,Fyb, Jka and Jkb antigens and see if I detect mixed field reactions. "with mycoplasma pneumoniae" I would like to see the results of direct agglutination test (immediate spin or room temperature) based on this diagnostics using Group O adult cells and cord cells. This antigram only includes IAT where anti-I may not be demonstrable. "which what exclusions and further testing would you perform" I would also like to see the DAT and Eluate from the cells (especially if I see mixed field reactions in my antigen typing) "When would you perform a titre ?" If the reaction with Group O adult and cord were both positive (tested at room temperature direct agglutination phase), I would perform titer using Group O adult and Cord cells in parallel to confirm the specificity of reaction seen in room temperature. Reactions greater than 3+. "If emergency units required with titre greater than 64 what is your protocol ?" I would make sure that transfusion is absolutely necessary by involving medical staffs. Lastly, I would like to perform ABO/Rh typing, obtain hematology test results (H/H, retic count, any abnormal RBC morphology?), Chemistry results ( Direct/Indirect Bilirubin, Heptoglobin), transfusion history (getting a list of hospitals that the patient has been to and calling each hospital has helped me alot in the past to get this information) on this patient, as it is an essential information in all cases of immunohematology investigation. Also, Drug-induced AIHA maybe a far fetch without further information, but something to be included in the back of my mind.
  2. I am just wondering if anybody (any Blood Centers) has been labeling units with historical antigen typings since 2017 FDA guidance came out. For those who are doing these, I am also curious to see what kind of processes you have in place and which antigens you are labeling using historical antigen typing. *FDA guidance document attached* UCM534978.pdf
  3. Most people would sacrifice the entire unit to perform allo-adsorption. In this case, most hospital blood bank would not be able to use up the entire unit fast enough so will be wasting some the adsorbing cells, if not kept frozen (as most hospital do not have a nitrogen tank or ultra low freezer). The other option to use donor unit is to gluderaldehyde-treat and freeze the stroma for later use. The other more feasible option is to type the lab (or blood bank) employees (of course with their consent) to have them as donors for adsorbing cells. When you have to perform adsorption you can use your staff's red cells as adsorbing cells. In this case, it will be an adsorption using the phenotype similar cells with your patient rather than a differential adsorption using 3 cells.
  4. I just answered this question. My Score FAIL  
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  8. dothandar

    BloodBankTalk: Allergic Reaction

    I just answered this question. My Score FAIL  
  9. dothandar

    Interview question: Hb7.1 or Hb 6.9

    are you allow to average it?
  10. dothandar

    High MCHC

    What is the diagnostics? Is there any indication of hemolytic anemia or abnormality in manual differential (if performed)?
  11. dothandar

    UK qualification and experience in USA

    I dont think you need special state licenses for these states. CLIA (Clinical laboratory improvement amendment) defines most tests performed in hematology and immunohematology as moderate to high complexity testing, therefore needs specific education/experiences or accreditation. The easiest way to achieve it is to challenge accreditation exams conducted by ASCP (american society for clinical laboratory science). I have the link to their website below. You can go ahead and make an account free of charge and start looking through education/experience requirements and paperworks needed to challenges the exams. As you have 17 years of prior experience, recommend you to look into BB(work in blood bank), MLS (can work in every area of the lab except for cytology), H (can work in hematology)which are technoligist licenses. Also, specialized licenses SBB (specialist in blood bank, which majority of blood bank lab leadership personnel are certified for) or SH (specialist in hematology, which majority of hematology lab leaderships are certified for). https://www.ascp.org/content/board-of-certification/get-credentialed Please do not hesitate to ask us questions here. We are all here to help you with your transition.
  12. dothandar


    This is an interesting thought. Chelating agents may or may not be the cause. The sources of plasma that I have worked with in this case are EDTA as well. It will be an interesting experiment to see if the serum and plasma from the same donors reacted the say way after being stored for a period of time.
  13. dothandar

    Billing for autologous frozen blood

    I would love to have a national system where all transfusion/transplant patients are treated equally regardless of their genetic basis, here, in the US. Unfortunately here in the States, blood is often times is looked as a commodity and treated as such, as well as the services that goes with it. I do not even want to start talking about how deficient our healthcare system is.
  14. dothandar

    Prewarm panel/screen

    we charge pre-warm tests as a screen or panel (depending on which one is performed)
  15. dothandar

    UK qualification and experience in USA

    It varies by the State that you are moving to. Majority of the US lab requires an ASCP certification at a minimum. May I ask which state you are moving to?

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