Walter Isenheim

Man you got me good, started searching for anti-O and anti-Q and thought that I missed something big It's cool, no hard feelings! Anti-P sounds much more familiar. That's why we don't do DL test ourselves, we don't have time for it.

You are like an encyclopedia Should of course have included some information about the patient. 67 year old woman with anemia. Suspected AIHA, but no established diagnosis. The patient has been moved to a larger regional hospital, so it is not certain we will get the final diagnosis. So "anti-O" and "anti-Q"? I opened my trusty antigen factsbook, but nothing… do you mind telling me more? We only perform DL-tests at the request of the department/patient responsible doctor, as we do not perform them in house. Did you perform DL-test routinely?

For me a somewhat unusual example, I would appreciate your opinions on it. 4C 37C Prewarmed Screen H 1+ neg Ak panel H 1+ neg Auto H 1+ neg With routine screen on gelcard (anti-IgG) at 37C all cells became 1+, NaCl tube test at 4C gave lysis with all cells, prewarmed on gelcard (anti-IgG) neg on all cells. Room temp 37C Forward A neg B 4+ Ctr neg Reverse A1 2+ neg B 4+ 3+ Extra reaction in reverse grouping in room temperature DAT IgG 1+ C3d 2+ Sample stored at 4C overnight gave extensive lysis and an unusable sample So a cold antibody that activates compliment, our laboratory rarely see lysis like this. How do you guys routinely investigate this? Do you do anything more? Cold titer? Recommending a blood warmer?

Thanks guys! I'm a Biomedical Scientist from Sweden. I work at Blekinge Hospital in Karlskrona and I'm in charge of the antibody identification laboratory. I have occasionally sneaked around the forum as a guest and finally decided to join. Will be interesting and I will hopefully have the opportunity to learn from people in the same profession. /Walter