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About rebeccarjthomas

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  • Birthday 02/06/1957
  1. We had a patient referred to our Ref Lab that had an anti-D reactive strongly in Solid Phase with all D+ cells but Ro cells. Customer was certain there was an unusual aby here.
  2. Yes -definitely get rid of the bleach! Also - bleaching plastic saline bottles may cause chemicals to leach out of the plastic - who knows how that would affect your testing!
  3. This is not an uncommon situation. For quite a few years, I was in charge of a prenatal testing lab at Memorial Blood Centers. One of the services we provided was Kleihauer-Betke testing. For quite a few years we repeated FMH screens on samples submitted for Kleihauer-Betke testing due to pos FMH screens when the Kleihauer-Betke test was negative. We followed the FMH screen procedure published by Betty Sebring, who, also, worked at Memorial. In all cases, the FMH screen test in our hands was negative.
  4. Quite a few of our customers use the ECHO. At times they report positive reactions, sometimes strong, that we cannot duplicate using manual Solid Phase. My understanding is that: On the ECHO - pt. sample is added prior to LISS. Manual Soloid Phase - LISS is added prior to patient sample. Has anyone looked at this difference specifically? I don't think I've seen anything published on manual versus ECHO Solid Phase testing. Thanks, Becky
  5. AIMS sponsored by SCABB in the spring. Great meeting!
  6. WHen using EGA treated cells for Weak D testing, we run a Weak D positive cell as a control. This is a cell that you can purchase. RJT
  7. Mike - DOn't you remember learning the Kleihauer technic all those years ago when you rotated thru Prenatal Lab at Memorial? Memorial has never read Kleihauers using oil. Hope you are well - give my regards to Marsha. You should get Dr. Megan to invite you to ICII. IT's in MN this year. Becky Thomas
  8. Wow- this sure sounds like age discrimination! What you really have is a long term training issue.
  9. Well how about when you suspect you may have a weak aby present - use ads. and elution to concentrate. (Adsorb a large volume of serum versus a smaller volume of cells say a ratio of 3:1 using an untreated adsorbing cell; elute back post adsorption to concentrate suspected weak aby).
  10. Well - there again I think you need to think about how sensitive the test method is that you are using. Some labs, for instance ARC IRLs, routinely rule out aby on heterzygous cells when testing in PEG, enzymes, GEL or Solid Phase.
  11. What about the possibility that the child was sensitized by Mom if Mom RH Pos? Jane Swanson had a person with, I believe anti-Fya, that the Mom seemed to be the only possible explanation for sensitizing event.
  12. INteresting. I worked for a long time at a facility where we repeated adsorption studies on patients with WAA every two weeks - recommending as compatible or more compatible than ac during the intervening time. However, we had a patient that developed a new antibody (anti-Kell) within one week post adsorption study. So at that time, we revised our policyon repeat adsorption studies to repeating once every seven days. Of course, one policy just like one technic serve as our best, thoughtfully considered practice - but exceptions happen - like your patient. Wow, did you just get a phenotype
  13. Malcolm, Say if you com eto MN (maybe for the ICII meeting next June) you could take a side trip up to northeastern MN. In Ely , gateway to the Boundary Waters Canoe Area, there is a fascinating well established Wolf Center. OR - you could read about it on the web. Maybe they would have some ideas to save "your" facility. Becky THomas P.S> I grew up in Ely.
  14. I agree - would be great Malcolm. Becky Thomas
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