globe

Yes, Agree. Doing so we validate the interpretation of result.

Guys Is any of you using NBM 200 non invasive hemoglobin meter for checking HB of Blood donors ? How do you do daily QC on this meter (literature attached), If vendor provide control how do you run on instrument. Thanks NMB200hemoglbinanlyzr.pdf

Dear khalidm3 Could you provide me the said " Transfusion, Service Manual of SOPs, Training Guides and Competency Assessment Tools 2nd edition From AABB" Really I would be very gratefull you.We realy need now. Regards Mohammed Salman Charge Technologist The Aga Khan University Hospital Blood Bank, Clinical Laboratory Stadium Road, P.O. Box 3500, Karachi 74800, Pakistan Phone: 92 213493 0051 Ext. 1596, Direct: 3486 1596 Fax: +92 3213493 4294 / 3493 2095. Email: muhammad.salman@aku.edu www.aku.edu

We are going to prepare CAP accreditation in a tertiary care hospital blood bank first time in our country,We need to prepare blood bank SOPs & Quality manaul according to Cap requirements. Pls provide soft copy to get the help tp prepare the SOPs and quality manual. Your cooperation would be be highly appreciated. globe e-mail: globes999@yahoo.com

Just want to be updates regarding this matter. Thanks SALMAN

Any procedure to remove C3d/C3b complements from Rbcs, Any PROCEDURE please !! SALMAN

Hi Malcom / akupaku Antigen e was negative on patient's pre transfusion sample. Rh Phenotype of patient was DCE/DCE, (Test was performed through the treatment of choloroquine diphosphate on patient's rbcs according to AABB) a positive control was also performed parallel with test .

Hi Malcom and aakupaku, We had performed the Antibody Identification with Immucor Gamma as well as with Diamed ID pannel, both were giving the result pattren of Anti-e more over earlier we had performed the Antibody screen with two said manufacturer and the result pattren were same.

Hi David & Malcom Thank you for guidence, Yes the Antibody Screening was positive with Diamed -ID cell aswell as with Immucor Gama panno Cell , For Phenotytyping of Le( we had used the BIORAD Antisera and used a ABpositive cells as positive control but not for a series. We had performed Allogenic adsorption with ZAAP treated cells and and after 4th adsorption , the screening and Identtification were performed, Anti-e Identified (Test performed with Pre-Transfusion sample) ,Xm were also performed we found 2 compatible units were found out of 37 units. Both units have been transfused and the hemoglobin is 5.9 g/dl. Thanks you very much.

A 20y male patient with known AIHA 6 yaear back , physician wan to transfused blood as the hemaglobin is 3.4 gm/dl . His initial serology was performed and the forward blood group is"B" Rh positive his reverse grouping showed agglutination with "A" cell but not agglutinated with "B" "O" cells. Direct Coomb test= 4+, Anti-IgG=4+ , Anti-C3d=negative , Auto Control= 4+ 50 units of pc of group B & O Rh positive & negative were Xm,all the unit were incompatible at coomb phase with Anti-IgG the rectivity were 3-4+ . At this stage we tested patient's cell for H antigen , the reaction was negative with Anti-H a swell as with Anti-Leb= negative. Is that para Bombay B along with AIHA or else ? Any suggestion regarding this please reply. Salman Charge Technologist Blood bank (AKUH) Karachi-Pakistan

Thanks Malcom

Malcom please e-mail a copy also to me at the following e-mail: muhammad.salman@aku.edu

we are planinng to discard CuSo procedure at our site, used to estimate the donor's hemoglobin .What are other economical / reliable alternative ? Any experience

Thank you David for providing very usefull material M.Salman

Hi Could anyone tell about the citation "Blood Donation waiting period in non-infectious Jaundice" from AABB/FDA. We have policy of waiting up to 4 years for "Jaundice".