Quite frequently, a cold autoantibody may not react on immediate spin (or demonstrate a positive reaction in the auto control), until the testing is dropped to a colder temperature. We often observe a "panagglutinin" (or often, reactions with some panel cells but not all panel cells, giving us a pattern that cannot be identified.) When we test the patient's plasma with screening cells and an auto control and incubate the tubes in the refrigerator for 15-30 minutes, then we see all the cells and auto giving 3+ or 4+ reactions (indicating the presence of a cold autoantibody.) Donna