[California - IS XM necessary when LIS can detect ABO incompatability?]

Is can detect anti-Vel which isclinical significant.

[Premature baby with Positive Antibody Screen?]

The elevated IgM in the baby means there was intrauterie infection. 

[Premature baby with Positive Antibody Screen?]

It seems like allo- anti-I. But I can't explain why the Liss reaction is neg. I am waiting experts to explain and learn from them.

[Antibody ID]

5 hours ago, Malcolm Needs said:

One thing that I would caution against, and that is diluting a "strong" antibody to make a "weak" antibody (although this is far more important when trying to make a "weak antibody" to use as a control (for example, for an IAT), as a "strong" antibody has a completely different equilibrium constant (although this may not be too important if you just use them for teaching).

May you kindly give us more details about it?  Is it about the ionic strength? Thank you.

[IS XM Positive-Next Step?]

On 12/22/2023 at 1:09 AM, REN_NH said:

Let's assume all units are reacting the same way due to a cold antibody.  What needs to be done to deem the units ABO type compatible?

Maybe repeat ABO typing can prove it.

[Urine Collection for a Transfusion Reaction]

It needs about 6 minutes to expel the substance in blood circulation through urine.

[Anti D level in 2nd pregnancy]

Just my guess, is there any possibilities of fetus D pos cells bind partof the antibodies?

[What problems in transfusion services that you encountered that is worth doing a study?]

We have encountered a patient who produced auto anti-A(or A1,sorry I didn't identify it).

[Cord Blood testing on gel]

Sorry,I can't find the English version.The  underlined sentence means the cord blood cells needed to be washed at least once before testing.

[Cord Blood testing on gel]

[Cord Blood testing on gel]

On 7/14/2023 at 9:05 PM, SbbPerson said:

I know this post is about 15 years old, but I recently came across a similar issue. I have always done DATs in tubes.  But currently switched to gel.  I am use to washing the cells when I do a DAT. I find it kind of odd that the package insert for the IgG cards doesn't include washing in it's procedure.  Do anyone know exactly why?   The insert said just to straight add 10uL of packed cells to 1.0 mL of your MTS diluent to get your 0.8% cell suspension.  

 

I got a weak positive reaction on a baby cord blood. I decided to wash the cells and the reaction came out stronger. Then I repeated this 2 more times and got similar results (see picture).  All controls were negative.

 

Has anyone experienced this ? And can I get your thoughts on this matter? Thank you so much, please have a nice day. 

 

 

 

 

I am so lazy that I just checked our IgG card today . I noticed it said that cord blood need to be washed before testing. I am at home now, I will attach the package insert tomorrow.

[Cord Blood testing on gel]

I guess this may be caused by the Wharton's Jelly which can cause rouleaux formation of the cord cells. For the gel technique, it is good to test the adult cells without washing, but for the cord cells, maybe the Wharton's Jelly block  antigens on the cells' surface. I noticed that patients suffer from Multiple myeloma will not show false positive reaction in gel, but saddly I have not confirmed if there are false negative reactons.

[Prenatal Antibody Titers]

I prefer the each  individual one policy. Because we can not make sure the baby has the combined antigens.

[Mock-up cases]

Thanks  for your sharing, Malcolm.

In the course of reading the cases above, I encountered  many problems which  resolved  by checking on books. This is a very  impressive way to absorb new knowledge. As a hospital blood bank worker, I rarely have chances to get access to thoes cases.  

 

[A 10month A pos baby with anti- A]

Here is a follow up. The baby had tested with anti-A on 27, April  and transfused with O washed cells the same day, but on 10, May she had no anti-A and received A type packed red cells with no transfusion reaction. She has stoped feeding on her mother's milk for 14 days.

I tried to persuade her mother to do some tests. The results came out and she has no anemia, with normal reticulocyte count and percentage. Her bilirubin and LDH are normal too. But she has not tested her haptoglobin.

She has not received any blood transfusion and IVIG.  She just diagnosed with slight anemia during pregnancy and after birth.  She had taken iron supplements during pregnancy,.

This is her eventh pregnancies and the third baby, she had several miscarriages. She told me her case seems like a mystery both to her and the doctors she know. She just want to know if she is ok and the baby will be healthy in the future.

 

[A 10month A pos baby with anti- A]

The baby is discharged now, we cannot test the mother anymore, what a pity.

[A 10month A pos baby with anti- A]

She is still breast feeding the baby,  and looks healthy. 

[A 10month A pos baby with anti- A]

Thank you very much, Arno. 

Only one question left, why does the mom  asymptomatic even with auto anti-A. We have encountered another case of auto anti-A, that one need transfusion.

 

[A 10month A pos baby with anti- A]

3 hours ago, Malcolm Needs said:

Sorry to ask this yan xia, as I have great respect for your knowledge, but are you absolutely certain that it is anti-A (or anti-A1), and not a rare case of anti-FORS1?

Thanks, Malcom.

1. I am not sure it is.anti-A, not.anti-A1. I mathed 5 A donors with the patient and the reactions.are all positive(Maybe they all A1). It is my fault, I need to add A2 cells to make sure about it.

2. I searched about the FORS1 online, there are few papers I can find. I will try my books tomorrow. This.is the.first time I read this antigen. Is there any cross reaction between it and A( or A1) antigen?

3. There is an idea just poped on my mind, it maybe Tn. But I don't know if Tn can give so strong reaction with monoconal anti-A reagent.

[A 10month A pos baby with anti- A]

12 hours ago, Cliff said:

Has the baby had any infusions / transfusion?  Plasma, platelets, IVIG?

No,  He has got nothing above. I guess the antobodies come from the mom, maybe from breast breeding , but have not heard about it before.  Will the milk origined  anotibodies come into the blood stream and be IgG?

[A 10month A pos baby with anti- A]

Thanks, sbbguy. At first I thought maybe the mom is A2 type, too. But she is A1 type with pos DAT due to IgG. I feel so confused.

[A 10month A pos baby with anti- A]

I have encountered a case, there was a 10 month baby boy, he was A type, but there was anti-A in his serum and on his red cells we also eluated anti-A.

His mom was A type with auto anti-A.

1.I remember the books say maternal origin antibodies will disappear 6 monthes after birth, maybe the books are lying😃 or there are some other reasons. He was breast feeded.

2.His mom looks healthy even with auto anti-A, but the baby developed hemolysis, why?

Thanks in advance for your help.

 

[Anti-D in O positive patient, DAT+, no RhIg given]

11 hours ago, L.C.H. said:

 

- RHD*weak partial 4.0  encodes p.201Arg and p.223Val

- Hybrid RHD*D111a-CE(4-7)-D does not encode D but encodes partial C as part of r'S haplotype

 

I was wondering why does  she express D pos, if the allele can not encode D?

[Transfusion Reactions:Hives]

On 6/26/2021 at 5:41 AM, cheru26 said:

Yes, the same unit.   once it is hung it is good for 4 hrs.    No need to use a new blood  product.    limit donor exposure. 

Thanks for your explanation.

I guess  there must be some measures be taken to prevent the opening system to be contaminated, would you please share it with me?

[Transfusion Reactions:Hives]

13 hours ago, cheru26 said:

The Dr. will stop the transfusion and administer benadryl an resume transfusion.  

For resuming transfusion, do you mean resume the blood which caused reaction or another unit of blood?