Reputation Activity
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psykobillys reacted to Ensis01 in Ortho to immucor reagentsQuestion to those wiser than I. Is the primary reason for these required validation comparisons to ensure we in the lab know how to follow the package inserts and so use the reagents correctly? I ask because I can't imagine any lab will be able to test the reagents to the level the manufacturer can with respect to numbers and variants (see package insert).
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I am retired now and will never forget the sound they make when they are dropped on the floor.
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psykobillys reacted to Bet'naSBB in Pipette usage for elutionUsing a new pipette each time is how I keep track of how many times I've washed!..........4 washes with Working Wash? - Lay out 4 pipettes!........AND close my drawer so I won't - out of habit - reach in and get one! LOL!
(I'm and "older tech" too! )
but as to your question........I don't know the true answer......... we always use a new one. But, if it does not state specifically in the IFU and their eluates / LW's give valid results.........I'd say it doesn't matter..........???
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psykobillys got a reaction from monopolova in anti-Jka likes to react unpredictably?I've seen it at least 2, maybe 3 times where reactions were consistent (ie repeatable) but didn't behave as expected - first of all, generally weak reactions, nothing stronger than a strong 1+ in gel, but reacted with some (not all) homozygous Jka cells and some (not all) cells that were heterozygous. each time, we threw more cells at it to prove/disprove the anti-Jka conclusion and each time the only consistency was that all of the reactive cells had the Jka antigen. could it have been some other antibody for which our panel cells didn't note the corresponding antigen? sure. but i wouldn't ignore the consistent presence of the Jka antigen on the reactive cells...
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psykobillys got a reaction from Mabel Adams in anti-Jka likes to react unpredictably?I've seen it at least 2, maybe 3 times where reactions were consistent (ie repeatable) but didn't behave as expected - first of all, generally weak reactions, nothing stronger than a strong 1+ in gel, but reacted with some (not all) homozygous Jka cells and some (not all) cells that were heterozygous. each time, we threw more cells at it to prove/disprove the anti-Jka conclusion and each time the only consistency was that all of the reactive cells had the Jka antigen. could it have been some other antibody for which our panel cells didn't note the corresponding antigen? sure. but i wouldn't ignore the consistent presence of the Jka antigen on the reactive cells...
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psykobillys got a reaction from Neil Blumberg in anti-Jka likes to react unpredictably?I've seen it at least 2, maybe 3 times where reactions were consistent (ie repeatable) but didn't behave as expected - first of all, generally weak reactions, nothing stronger than a strong 1+ in gel, but reacted with some (not all) homozygous Jka cells and some (not all) cells that were heterozygous. each time, we threw more cells at it to prove/disprove the anti-Jka conclusion and each time the only consistency was that all of the reactive cells had the Jka antigen. could it have been some other antibody for which our panel cells didn't note the corresponding antigen? sure. but i wouldn't ignore the consistent presence of the Jka antigen on the reactive cells...
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psykobillys got a reaction from John C. Staley in anti-Jka likes to react unpredictably?I've seen it at least 2, maybe 3 times where reactions were consistent (ie repeatable) but didn't behave as expected - first of all, generally weak reactions, nothing stronger than a strong 1+ in gel, but reacted with some (not all) homozygous Jka cells and some (not all) cells that were heterozygous. each time, we threw more cells at it to prove/disprove the anti-Jka conclusion and each time the only consistency was that all of the reactive cells had the Jka antigen. could it have been some other antibody for which our panel cells didn't note the corresponding antigen? sure. but i wouldn't ignore the consistent presence of the Jka antigen on the reactive cells...
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psykobillys reacted to Neil Blumberg in anti-Jka likes to react unpredictably?Yes. And worse, some cells react differently, including having no reactivity, as compared with cells of the same degree of zygosity. Thus the possibility of Kidd antibodies needs to be seriously considered when the recipient is negative for one or both antigens and the panel is reactive but without clearcut specificity for Jka or Jkb.
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psykobillys reacted to Malcolm Needs in anti-Jka likes to react unpredictably?I worked in Red Cell Immunohaematology for most of my 43 years before retirement, including two times at the International Blood Group Reference Laboratory (IBGRL), and for over a decade at one of the NHS Blood and Transplant Centres in London. During that time, I saw some pretty weird Kidd antibodies, but never came across an example of one that reacted with red cells with Jk(a) heterozygous expression, but not with Jk(a) homozygous expression.
One such "weird" type (although I never saw one) was the extremely rare, dominant inhibitor type In(Jk), similar, but, of course, not identical to In(Lu). These red cells usually type as Jk(a-b-), but their true Kidd type can be ascertained by Adsorption and elution tests.. These red cells are also more resistant to haemolysis by 2M Urea than red cells with "normal" expression of the Kidd antigens, but less resistant to haemolysis by 2M Urea than true "amorphic" Jk(a-b-) red cells.
There are approximately 14, 000 copies of the Kidd carrier molecule per red cell (quite a small number, when compared with some other carrier molecules, such as the D antigen).
The amino acid residue that defines either the Jka or Jkb antigens is very close to the red cell membrane in the 4th extracellular loop but is largely “hidden” by the 3rd extracellular loop (steric hindrance).
Both facts may contribute to the weak reactions between Kidd antibodies and Kidd antigens.
Schematic of the Kidd carrier molecule (after Wester ES, Storry JR, Olsson ML. Characterization of Jk(a+weak): a new blood group phenotype associated with an altered JK*01 allele. Transfusion 2011; 15: 380-392. DOI: 10.1111/j.1537-2995.2010.02795.x.
In this paper, Wester et al also describe weakened forms of both the Jka and the Jkb antigens, but in each case, the amino acid substitution is remote from position 280 of the mature protein.
In addition, an individual with the Trp171Arg mutation with weak Jk(a) expression has produced an anti-Jk3 or anti-Jk3-like antibody, and so they may be “dangerous patients” (Whorley T, Vage S, Kosanka J, Lose SR, Sandquist AR, Copeland TR, Westhoff CM. JK alleles associated with altered Kidd antigen expression. Transfusion 2009; 41 (Suppl.): 48A-49A (abstract).
Lastly (for now anyway!), most foetal red cells sensitised by maternal antibodies react only with anti-IgG, but I (and a colleague Grant Webb) have both noticed, but not published, occasions when such red cells also react with anti-C3d and, in one case, only anti-C3d (see genuine photographs below)..
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psykobillys reacted to Malcolm Needs in anti-Jka likes to react unpredictably?I should have also said that there is an excellent Blood Group Review available, viz Hamilton JR. An update to Kidd blood group system. Immunohematology 2024; 40: 28-33. DOI: 10.2478/immunohematology-2024-005.
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psykobillys got a reaction from SbbPerson in BloodBankTalk: PR platelets and septic transfusion reactionsI just answered this question.
My Score PASS
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psykobillys got a reaction from Malcolm Needs in BloodBankTalk: Correct Blood Bank NomenclatureI just answered this question.
My Score PASS
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psykobillys got a reaction from Malcolm Needs in BloodBankTalk: Correct Blood Bank NomenclatureI just answered this question.
My Score PASS
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psykobillys got a reaction from SbbPerson in BloodBankTalk: QC of multiple reagent racksI just answered this question.
My Score PASS
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psykobillys got a reaction from Cliff in Welcome psykobillysHello!
I've been in the laboratory field for about 20yrs and have been in the Blood Bank for about 10yrs.
I've never posted here but have definitely used the site for guidance many times!
Thank you!
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psykobillys reacted to applejw in product modification labeling requirements...If you are not changing the component label, doesn't the storage temperature also need to be modified in addition to the expiration date/time?
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psykobillys reacted to jayinsat in product modification labeling requirements...You are in a tough situation. Either your facility will need to invest in a digitrax printer to print ISBT labels or you should not modify the component in the LIS, only manually change the expiration date on the face label and document the new expiration after issue and transfusion (or by comment). I do not like the former option. I would insist on obtaining an ISBT label printer if I were in your position. That is the cost of having a blood bank.
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psykobillys reacted to tcoyle in product modification labeling requirements...Per the United States Industry Consensus Standard for the Uniform Labeling of Blood and Blood Components Using ISBT 128 Version 3.0.0 March 2013 published by ICCBBA, for thawed plasma products or Cryo AHF, thawed plasma products that are used with the 6-24 hour expiration can keep their original product code. The expiration date and time must be changed and that information does not need to be bar coded. The original expiration date bar code should be lined thru, date and initialed. You can then write the new expiration date and time below that.
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psykobillys got a reaction from Malcolm Needs in Welcome psykobillysHello!
I've been in the laboratory field for about 20yrs and have been in the Blood Bank for about 10yrs.
I've never posted here but have definitely used the site for guidance many times!
Thank you!