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Monique reacted to a post in a topic: Alternative to DTT treating cells for ABSc when patients are treated with anti-CD38
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LISS Validation?
We switched to N-Hance years ago in order to comply with Massachusetts Water Resources regulations. ImmuAdd contains the preservative thimerosol-a mercury contaminant. We performed a small validation and found that N-Hance performed as well as ImmuAdd. Only one problem: the manufacturer's insert suggests using a transfer pipet to dispense the additive rather than the vial dropper in order to maintain the proper ionic strength. When we open a vial, we always discard the dropper and replace it with a stopper.
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Weak D Testing - Cord Blood Evaluation
Limitations to the FHM RapidScreen Test 1. For correct interpretation of the test results, the test must be performed on the blood of a known D-negative mother of a recently delivered D-positive child. If the infant's red blood cells possess a weak D antigen or partial D antigen, the test may not detect a fetomaternal hemorrhage exceeding 30 mL of whole blood.
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Units being returned from isolated patients
We assume that every patient is a COVID patient. We place each unit in a zip lock bag with the unit tag placed along the back of the bag, seal the zip lock and place a label across the top of the seal warning that if the seal is broken the unit will be discarded. We do this for every unit.
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MD signature on orders
Do you require a MD signature on non electronic blood bank orders or is a name satisfactory?
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Strange Blood Type Discrepancy
Thank you for making my day
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Routine Testing on Newborns
We receive cord samples on every baby and only test the ones (ABO/Rh DAT) from Rh negative moms or those with a clinically significant antibody. Our LIS allows us to see both mom and baby demographics at sample log-in. We never perform an eluate from the cord.
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Strange Blood Type Discrepancy
How about cis AB?
- Psoralen treated platelets
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Anti-Bg and Capture
We are suddenly detecting anti-Bga using Capture solid phase technique. What gives? Is anyone else picking up these nuisance antibodies in Capture (they are not reproducible in PEG/IgG)?
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SBB project ideas 2013
How about a nationwide survey gathering information on how hospitals prepare aliquots for neonatal transfusion. Do they have an anticoagulant preference? Do they use a dedicated unit or select only fresh blood? Do they issue the unit in a syringe or in a pedi-bag? Are the units CMV negative or leukoreduced? Practice seems to so varied that it would be nice to get an overview and perhaps best practice ideas.
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Wise Ole Sayings
If you're in a hole, stop digging
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Misidentification from Reference Lab
It well documented and if you take a look at Issitt's Blue Bible "Applied Blood Group Serology" you see numerous citations where certain serums contain loads of antibodies to low freqs--the Blo serum contained antibodies to Bpa, Gf, Jna, Moa, Or, Pta, Ria, Swa, Tra, Vga, Vw, Wra, BOW ans Skjelbred--a real hodge podge of goodies
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How to transfuse T-active patient?
here's a link to the California Blood Bank Society where they addressed this topic http://www.cbbsweb.org/enf/2004/ffp_lowtiter_anti_t.html
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When do you do Fetal Screen?
We only perform a FMH test(Rh positive infants, negative Mom) postpartum. The Gamma/Immucor kit insert does not recommend testing infant's possessing a weak D antigen sine the test may not detect a FMH exceeding 30 ml. We send all other requests to the Heme Lab for a KB
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Pooling Cryo and Re-freezing
With a very busy liver program and active emergency room, we find it very helpful to have prepooled cryo on hand-especially for our overnight crew-pooling a cryo while you're crossmatching, thawing plasma, tagging a platelet, (maybe preparing factor 7a) packing coolers and answering the phone is always fun. I can forward you our SOP. FDA did review our validation documents and gave us their blessing to proceed-we routinely keep 15 frozen pools on hand at all times.