sherif 6 Posted May 29, 2018 Hello About ORTHO gel card ( for blood grouping and AHG ) can we spin it in Diamed centrifuge (10 minutes ) or this will affect the result ( false positive /false negative )? Share this post Link to post Share on other sites
galvania 762 Posted May 30, 2018 Short answer - No, you can't 1 BBNC17 reacted to this Share this post Link to post Share on other sites
sherif 6 Posted May 30, 2018 8 hours ago, galvania said: Short answer - No, you can't Thanks but why ? Share this post Link to post Share on other sites
SMILLER 860 Posted May 31, 2018 It may be seen as modifying Ortho's method, which means you would a have to have a great deal of data to validate it. On the other hand, if you can get Ortho to state that it is equivalent to their centrifuges (which may actually be made by Diamed!), then it seems like it would be OK. Scott 1 David Saikin reacted to this Share this post Link to post Share on other sites
sherif 6 Posted May 31, 2018 5 hours ago, SMILLER said: It may be seen as modifying Ortho's method, which means you would a have to have a great deal of data to validate it. On the other hand, if you can get Ortho to state that it is equivalent to their centrifuges (which may actually be made by Diamed!), then it seems like it would be OK. Scott Thanks Share this post Link to post Share on other sites
galvania 762 Posted June 1, 2018 The reactions you will see in gel cards are based on the ability of un-agglutinated cells to pass through the material in the column to get to the bottom; and agglutinated cells to be retained. The centrifugation speed and the time of centrifugation are carefully calibrated so that that happens. But that is specific to factors such as the size of the card, the weight of the card, and numerous factors relating to the contents of the well (type of gel/beads etc) and everything you add to the well. So as the two cards are not the same, the parameters will need to be different as well. 2 Malcolm Needs and yan xia reacted to this Share this post Link to post Share on other sites
galvania 762 Posted June 1, 2018 Plus, if you have problems, neither Ortho nor DiaMed (now BioRad) would give you any support I guess 3 SMILLER, Malcolm Needs and exlimey reacted to this Share this post Link to post Share on other sites
sherif 6 Posted June 1, 2018 8 hours ago, galvania said: The reactions you will see in gel cards are based on the ability of un-agglutinated cells to pass through the material in the column to get to the bottom; and agglutinated cells to be retained. The centrifugation speed and the time of centrifugation are carefully calibrated so that that happens. But that is specific to factors such as the size of the card, the weight of the card, and numerous factors relating to the contents of the well (type of gel/beads etc) and everything you add to the well. So as the two cards are not the same, the parameters will need to be different as well. 8 hours ago, galvania said: Plus, if you have problems, neither Ortho nor DiaMed (now BioRad) would give you any support I guess Thanks alot Share this post Link to post Share on other sites
