Posted December 23, 201410 yr comment_58698 Hello, I currently use Gel as my primary method. A tech on the weekend got the following: 3 cell screen: 2+ for allGel panel A (11 cells) 1+ and 2+ reactions with the exception of 2 cells and the Auto control that were neg.Gel panel B (11 cells) 1+ and 2+ reactions with the exception of 3 cells that were neg. No pattern of single, multiple, multiples with dosage. Wells appeared to have thin sheet on the top of the column. Tube method immediate spin revealed Rouleaux and AHG results were negative. Saline Rep was done at immediate spin and reactions were removed. Is this a good enough, to prove that Rouleaux is causing the reactions in Gel? What would you recommend to indicate reactions are Rouleaux and not something else? Thanks, -Serafin
December 24, 201410 yr comment_58718 When we used gel and had a problem like that we repeated the screen with tube. If the tube screen was negative, that's what we reported, but noted the problem with the gel testing.
December 24, 201410 yr Author comment_58724 OK, that's what we've done so far.I was just wondering if that is evidence enough to show that there are no clinically significant antibodies or we should've done a panel as well.
December 25, 201410 yr comment_58736 I agree with AMcCord, you have proven that there is no clinically significant antibodies and that rouleaux is the culprit in the gel reactions.
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