Heat elution to detect Rh HDN

[Yanxia]

In our lab , we want turn to heat elution ( 56 degree C water bath for 10min) to detect our HDN specimen.

 I know it is good to detect ABO HDN, but for Rh and other group HDN it is not so sensitive. But we have limited resource .

I want to know whether I can use only heat elution to detect all HDN, if some specimen is DAT strong pos, we turn it to ref lab.

[Malcolm Needs ☆]

I can assure you Yanxia, that when I started at the Blood Group Reference Laboratory (way back in 1973), we used to use heat elution (56oC) and it worked fine!

 

Incidentally, I would recommend the Lui technique for ABO HDFN.

[simon hook]

I can back you up there Malcolm, although I only started in 1976

[David Saikin]

Yes, you can detect anti-D with heat.