JoyG Posted January 21, 2014 Share Posted January 21, 2014 Just a quick question. We have a Jkb which is not reacting with the plasma from the purple top but it is reacting with the serum in the red top which I would expect due to complement in the red top and not the purple but would it still react with Anti-IgG? We did not use polyspecific. Patient had a previous antibody, we ran out of plasma and went to the red top tube and low and behold the Anti-Jkb pops up reacting with IgG antisera. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted January 21, 2014 Share Posted January 21, 2014 Yes, this is quite normal. Kidd antibodies, as a whole, tend to be IgG, a mixture of IgG and IgM, or just IgM, but the IgG part of the mixture tends to be IgG1 and/or IgG3 (sub-types of IgG that are "good" at setting off the classical complement pathway), so, if the antibody has become labile in vivo and in vitro using anti-IgG, you have more chance of detecting it with a complement detecting AHG. However, monospecific anti-IgG is more sensitive for IgG antibodies than is polyspecific AHG, and so, once again, yes! rravkin@aol.com and JoyG 2 Link to comment Share on other sites More sharing options...
Dr. Pepper Posted January 23, 2014 Share Posted January 23, 2014 Malcolm, am I missing something here? You give a nice description of the differences in reactivity one can obtain with polyspecific AHG vs. monospecific anti-IgG, but JoyG used the monospecific in both cases. The variable was serum vs. EDTA plasma. I would think that when using monospecific anti-IgG, having active complement or not would be a non-factor. Link to comment Share on other sites More sharing options...
galvania Posted January 24, 2014 Share Posted January 24, 2014 Silly question - what is in the purple top tubes?Thanksanna Link to comment Share on other sites More sharing options...
David Saikin Posted January 24, 2014 Share Posted January 24, 2014 EDTA specimen - we call them lavendar tops, some are pink also. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted January 24, 2014 Share Posted January 24, 2014 Sorry Phil, I've had a very bust couple of days. I WILL reply. Link to comment Share on other sites More sharing options...
galvania Posted January 28, 2014 Share Posted January 28, 2014 Well then that is a bit wierd. I can quite get how you could get a reaction from serum and not EDTA-plasma if you are working with polyspecific Coombs, but I don't see how it would make a difference on anti-IgG only. The only thing I can think is - (always presuming that the 2 specimens REALLY come from the same patient) - did you do the identification panel on the EDTA and then the crossmatch with the serum? Or different types of cell? And how positive was the positive result - strong, weak or 'there if you're really looking for it'?Anna Yanxia 1 Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted January 29, 2014 Share Posted January 29, 2014 Sorry (particularly to Phil) for taking so long to "re-reply", but I have been up to my eye balls over the last few days. Yes, I agree that my first response did not make a lot of sense! What I meant was that although the EDTA (purple top) would have negated the complement, if you are using a red top (serum), you have "two bites of the cherry", because the anti-Jkb is likely to be a mixture of IgG and IgM. In such a situation, you have a case where the IgG bit of the anti-Jkb would be detected by the monospecific anti-IgG, but the IgM AND the IgG (given it was IgG1, IgG3 or a combination of IgG1 and IgG3) would, PERHAPS have led to the situation where the IgG and the IgM (which would also activate complement) would potentiate the agglutination of Jk(b+) red cells by "addition" of the factors. OF COURSE, ALL THIS IS SPECULATION, AND I COULD BE TALKING COMPLETE NONESENSE! Link to comment Share on other sites More sharing options...
galvania Posted January 30, 2014 Share Posted January 30, 2014 Um Malcolm the only thing is that usually the anti-IgG in the polyspecific Coombs is the same as the the anti-IgG in the anti-IgG reagents, and unless they specifically say otherwise, both would cross-react with IgM.And you would have the same amount of IgG and IgM in both EDTA and clotted blood, wouldn't you? Or have I misunderstood? I still think that there was a second, as yet undiscovered, factor that is responsible for this difference Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted January 30, 2014 Share Posted January 30, 2014 Yes, I think you are right on both counts Anna. I shall now go into hibernation for the Winter!!!!!!!!!!!!!!!!!!!!! Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now