Anti-A 0 but Anti-A1: 2+

[yanhan]

 A BLOOD DONOR

Anti-A:  0    Anti-B:  4+

 

A1 Cells:  w+     B cells:     0      O cells:  0

 

Anti-A1:  2+ (mixed field)

 

Any sugestions?

[Yanxia]

It is interesting. DO you have pos and neg control to ensure the reaction is reliable?

[Dr. Pepper]

How about subgroup A(lae)? Correction: Oops - just noticed the 4+ with anti-B. I should read this better (or slower).

Edited November 11, 2013 by Dr. Pepper

[galvania]

What exactly are you using as reagents?  And what method are you using?

[yanhan]

pos and neg control no problem

method is tube test

anti-A  anti-B  are monoclonal reagents  

results of two manufactures (immucor and diagast)  are  same.

Anti-A1 lectin is immucor regent  

 

Why anti-A neg ,anti-A1 pos?  

Many thanks

[Malcolm Needs ☆]

Possibly a Cad+?

Have you tried the patient's red cells against any other human-derived polyclonal antibodies? The red cells may be polyagglutinable.

[David Saikin]

You aren't reading the lectin under the microscope are you?  It is a macro only read. 

[Auntie-D]

What is it using gel?

[Corriedog]

What happens when the reverse grouping cells are incubated at RT for 15 to 30 minutes and /or cooled to 4^oC?

What is the age of the patient?

[aafrin]

Why did you test it with A1-lectin in the first place?

[galvania]

I think you need to do some molecular biology.  I guess you have a weak A subgroup.  Most peculiar that it's coming up with the lectin and not the clones, but that could be something to do with the formulation of the reagents.  There might be some potentiators in the lectin that aren't present in the clones, or the pH could be different etc etc. I agree with Malcolm that you need to test with human anti-A - tube technique.  Use an excess of anti-A (if reagents aren't available, use group B plasma) and incubate at 4°C and (separately) at RT.  That should give you some answers