Validity of combs check cells

[Desoki]

Dear colleagues,

I want to know how many days' cells still valid after manual preparation of combs' check cells by isotonic saline method?

[Liz0316]

not that I've had to do this in a while, but I don't think I'd make cells up for anything that were over 7 days old. And, then I would give them a 7 day outdate. I have no reference for this, but I think it's what we used to do in the past.

Liz

[David Saikin]

If you are making complement positive cells, I would only make them up for day of use, 2 days at most.

[Dansket]

I agree with David, but a bit more stringent. Red cells stored in saline at 4C are good for 24 hours maximum.

[rravkin@aol.com]

Just curious to know if you have a reference for your statement. I have always thought that isoton is very viable solvent for rbc's, but maybe 24 hours is truely a long time?

I agree with David, but a bit more stringent. Red cells stored in saline at 4C are good for 24 hours maximum.

[Dansket]

Just curious to know if you have a reference for your statement. I have always thought that isoton is very viable solvent for rbc's, but maybe 24 hours is truely a long time?

Plain saline has nothing for the red cells to feed on. See page 874-875 of the 16ed of the AABB Technical Manual under heading Method 1-6. Preparing a 3% Red Cell Suspension. See note at the bottom left of page 875.

[galvania]

I think you need to validate this yourselves in your own laboratory. You need to compare the stability of the reactions over a period of time and repeat this a number of times until you are sure that you can say that they can be used for x days. You also need to check for haemolysis in your system and see how long they keep and under which conditions. Why don't you just buy them?

[Desoki]

Why don't you just buy them?

Sometimes there is shotage of supply

[Eagle Eye]

I would go with what Ana said. If your validation shows stability of 10 days I would keep 7 days (on conservative side). We make positive DAT specimen for ProVue & manual gel QC and when we validated the vlidation showed 10 days and we use it fro 7 days.

I think you need to validate this yourselves in your own laboratory. You need to compare the stability of the reactions over a period of time and repeat this a number of times until you are sure that you can say that they can be used for x days. You also need to check for haemolysis in your system and see how long they keep and under which conditions. Why don't you just buy them?