We use a saline method where by we use 4 drops of plasma to one drop cells, to super-saturate the cell. Incubate 30- 60 min and use IgG at coombs.
This method has served us well in patients with warm autos. Malcom, of course, went into detail about strong bonds and titers, etc., but I tell my techs that "any self respecting allo- antibody will be detected by this method" -
and yes, I'm old school. So it was, back then, once you have discovered there is a problem, or you have actually detected an antibody, going back to a saline method is a fine and accepted way to get around the garbage you may be detecting in GEL or other "enhancing" method.
Liz