californiacls Posted March 21, 2012 Share Posted March 21, 2012 Hello Pathlabtalk communitiy,I am new to this site. I have a question. I received a cord blood sample that I was not sure what to type as. Here was my work. Any help would be greatly appreciated. ThanksBaby's cord blood was tested from a O Neg mom. Initial cord testing came to A Neg with weak positive DAT. Weak D was performed: Cord=negative, control with 6% albumin=microscopic agglutination What is the correct Rh to the baby RBC? Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted March 21, 2012 Share Posted March 21, 2012 Welcome to this fantastic site.In terms of giving blood to the baby, then regard it as D NEGATIVE until proven otherwise.In terms of whether or not the Mum needs anti-D immunoglobulin prophylaxis, then regard it as D POSITIVE until proven otherwise.I think there is a fair chance that the baby really is D negative, and what you are detecting is maternal IgG anti-A on the baby's red cells. Link to comment Share on other sites More sharing options...
californiacls Posted March 21, 2012 Author Share Posted March 21, 2012 Thank you. Malcom Needs. I appreciate your input.Love this site. Will visit regularly for real life work troubles I may encounter again in the future. Link to comment Share on other sites More sharing options...
Likewine99 Posted March 21, 2012 Share Posted March 21, 2012 I agree w/Malcom 100%. In this instance I have seen this reported as "unable to determine Rh type due to positive DAT". Bet the DAT will resolve itself eventually. Link to comment Share on other sites More sharing options...
Mabel Adams Posted March 22, 2012 Share Posted March 22, 2012 Didn't californiacls say that the weak D test with the cord blood was negative and only the control was microscopically positive? I would be pretty willing to call this D neg as long as the check cells worked. I have seen weakly pos DATs elute off in the weak D test making it possible to interpret the test even though a pos DAT usually would make the weak D test invalid. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted March 22, 2012 Share Posted March 22, 2012 You could always treat the baby's red cells gently with choroquine diphosphate and then see what results you get. Link to comment Share on other sites More sharing options...
David Saikin Posted March 22, 2012 Share Posted March 22, 2012 I always liked a heat elution on those weak DAT+ cords. They always cleaned up nicely. Need a 56C water bath. Link to comment Share on other sites More sharing options...
L106 Posted March 23, 2012 Share Posted March 23, 2012 Didn't californiacls say that the weak D test with the cord blood was negative and only the control was microscopically positive? I would be pretty willing to call this D neg as long as the check cells worked. I have seen weakly pos DATs elute off in the weak D test making it possible to interpret the test even though a pos DAT usually would make the weak D test invalid.I am comfortable with Mabel's logic on this.Donna Link to comment Share on other sites More sharing options...
swede Posted March 23, 2012 Share Posted March 23, 2012 Our docs like to have a heelstick done on the baby so we can do another type. They typically resolve to being Rh negative. Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now