snilon Posted July 14, 2010 Share Posted July 14, 2010 Does anyone have a recipe for making in-house QC material to QC complement reagent?We use C3b-C3d. I know you can purchase QC but wondered if anyone made their own.Thanks ! Link to comment Share on other sites More sharing options...
Eoin Posted November 23, 2010 Share Posted November 23, 2010 Too hard.We buy in sensitised cells from Immucor I think. Cheers Eoin Link to comment Share on other sites More sharing options...
David Saikin Posted December 17, 2010 Share Posted December 17, 2010 Quotient Biodiagnostics (Alba Scientific across "the pond") is working on a kit which will allow you to prepare your own. Not available in the US - I don't know about "over there". I heard this on last Friday from there US Technical Specialist. Link to comment Share on other sites More sharing options...
Eagle Eye Posted January 20, 2011 Share Posted January 20, 2011 what do you use as negative control for Ortho anti-C3b,C3d reagent.....package insert recommends using "The negative control is a test with washed, enzyme-treated red cells"Thnaks Link to comment Share on other sites More sharing options...
Eagle Eye Posted January 20, 2011 Share Posted January 20, 2011 These might be stupid questions but I am goign to ask anyway:how do you run your QC for anti-IgG &/or anti-IgG,anti-C3d reagents...do you wash it three times and then add your IgG reagent? do you add check cells to your negative control???Thank you Link to comment Share on other sites More sharing options...
David Saikin Posted January 20, 2011 Share Posted January 20, 2011 I do my testing in gel . . . buffered gel card for anti-C3b,-C3d. For neg ct I run the pt cells with diluent. Link to comment Share on other sites More sharing options...
David Saikin Posted January 21, 2011 Share Posted January 21, 2011 I may have misunderstood this . . . my patient test is my negative control (unless it is positive - very rare for my institution).I do my testing in gel . . . buffered gel card for anti-C3b,-C3d. For neg ct I run the pt cells with diluent. Link to comment Share on other sites More sharing options...
fenwayman Posted January 21, 2011 Share Posted January 21, 2011 Immucor has a great C3b,C3d control! Link to comment Share on other sites More sharing options...
Eoin Posted January 24, 2011 Share Posted January 24, 2011 We use the Immucor control cells - They provide Positive only - but like David, we use a patient with no histroy or symptoms as a negative (usually someone in for elective surgery group, screen & hold).CheersEoin Link to comment Share on other sites More sharing options...
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