ecd 0 Posted February 17 Share Posted February 17 I know we're a little late to this but I'm trying to write a procedure/validate using DTT to eliminate Dara interference using the premade 0.2M DTT from HemoBioscience and was wondering if anyone that's currently performing the testing would be willing to share their SOP. At first glance it seemed so simple: thaw, treat the cells, test them- done. I think we all know nothing is that simple though, so the more I work on it the farther down the rabbit hole I go (what temp does it thaw at, how important is a specific pH of 7.3 on this PBS really, how should I label this stupid stuff to keep people from thawing it too many times, etc). So if anyone is willing to let me look at how you're doing it I would really appreciate it. Thanks! Link to post Share on other sites
carolyn swickard 219 Posted February 18 Share Posted February 18 TS- Dithiothreitol -DTT- Treatment of RBCs.pdfThis is our procedure for the HemoBioScience product. it will be open for 30 days only. (I think) Don't worry about thawing it too many times - there is only 2-4 mls in each tube, so it doesn't last for that many pts. We have just thawed ours at room temp. We wrote the procedure using both the HemoBioScience procedureand the one in the AABB Tech Manual. Best of luck Henrique and AMcCord 1 1 Link to post Share on other sites
ecd 0 Posted February 19 Author Share Posted February 19 Thanks Carolyn! It's definitely helpful. Do you also use DTT to treat plasma to differentiate between IgG and IgM? I'm thinking since we're going to have the reagent we might as well add that to our options too but I'm not sure it's worth it. One step at a time I guess lol. Link to post Share on other sites
Cliff 436 Posted February 21 Share Posted February 21 Preparation and Testing of DTT Treated Plasma.docx AMcCord 1 Link to post Share on other sites
carolyn swickard 219 Posted February 22 Share Posted February 22 On 2/19/2021 at 7:28 AM, ecd said: Thanks Carolyn! It's definitely helpful. Do you also use DTT to treat plasma to differentiate between IgG and IgM? I'm thinking since we're going to have the reagent we might as well add that to our options too but I'm not sure it's worth it. One step at a time I guess lol. No - we do not have that procedure and I do not anticipate adding it. Link to post Share on other sites
NicolePCanada 23 Posted Friday at 12:56 PM Share Posted Friday at 12:56 PM Our DTT procedure is send it to a reference lab. I can't help out with this one. Sorry. Link to post Share on other sites
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