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Anyone want to share a DTT procedure?


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I know we're a little late to this but I'm trying to write a procedure/validate using DTT to eliminate Dara interference using the premade 0.2M DTT from HemoBioscience and was wondering if anyone that's currently performing the testing would be willing to share their SOP.  At first glance it seemed so simple: thaw, treat the cells, test them- done. I think we all know nothing is that simple though, so the more I work on it the farther down the rabbit hole I go (what temp does it thaw at, how important is a specific pH of 7.3 on this PBS really, how should I label this stupid stuff to keep people from thawing it too many times, etc).  So if anyone is willing to let me look at how you're doing it I would really appreciate it.  Thanks!

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TS- Dithiothreitol -DTT- Treatment of RBCs.pdfThis is our procedure for the HemoBioScience product.  it will be open for 30 days only.  (I think)

Don't worry about thawing it too many times - there is only 2-4 mls in each tube, so it doesn't last for that many pts.  We have just thawed ours at room temp.  We wrote the procedure using both the HemoBioScience procedureand the one in the AABB Tech Manual.

Best of luck

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Thanks Carolyn! It's definitely helpful.  Do you also use DTT to treat plasma to differentiate between IgG and IgM?  I'm thinking since we're going to have the reagent we might as well add that to our options too but I'm not sure it's worth it.  One step at a time I guess lol.

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On 2/19/2021 at 7:28 AM, ecd said:

Thanks Carolyn! It's definitely helpful.  Do you also use DTT to treat plasma to differentiate between IgG and IgM?  I'm thinking since we're going to have the reagent we might as well add that to our options too but I'm not sure it's worth it.  One step at a time I guess lol.

No - we do not have that procedure and I do not anticipate adding it.

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