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Everything posted by AuntiS

  1. We do a screen and, if indicated, A and B cells. sandra
  2. We didn't label the freezer drawers. But we do have a job aid hanging on the door with the freezer contents (by drawer). It makes it much easer if/when you move your contents around. sandra
  3. We do similar to labguru. We went from manual tube to automated gel (BioRad) and have found the same thing. If we have a discrepancy we send it out for genotyping. Most come back as a weak D type 1 (or 2 or 3). I am considering doing a manual tube type on our females less than 45 when they type as Rh positive the first time. And then sending out any discrepancies for genotyping. sandra
  4. Thank you for the feedback! We are pretty happy with the Bio-Rad cards and IH-500 as well. I'm still trying to figure out how to automate the antigen typing process with the QC available (needing a single dose for the positive QC) but feel like for our core lab staff using column agglutination for antigen testing has been widely preferred. sandra
  5. In Canada, our standards also add a requirement to clearly labelled/segregated area: CSTM Contamination of blood components or blood products from patient samples, reagents and/or tissue products shall be avoided by ensuring that blood components and blood products are stored in designated storage equipment or in clearly labelled segregated areas within the storage equipment. (See guidance statement below: Physical barriers are needed to prevent contamination of blood components and blood products from other materials stored in the same equipment or area. Examples of physical barriers include a leak-proof shelf or container (preferably with a lid), clearly labeled to reflect the contents. If the physical barrier is a shelf, blood components/products should be stored above any potential contaminants (reagents, patient samples, etc.).) sandra
  6. We do the same using the same reagents (but called Bio-Rad) and the SAXO (similar to the Banjo, i think). Our reagents are all scanned into the IH-Com when using the SAXO reader - as long as the IH-500 uses the same reagents the QC flag is absent. sandra
  7. We use an IH-500. If the reactions in the software have no discrepancies (from previous or within the current results, weak reactions, error codes, etc.) they will autovalidate to our LIS. Our LIS does not autoverify. Those are always looked at before being released. sandra
  8. Wow, reading the challenges my American colleagues are having! I thought it was bad here in Ontario, Canada. The hospital laboratories here generally pay the same - unionized or non. And we don't generally have any sign on bonuses outside of working in the North, although I have been hearing some nursing/physician incentives. And I don't think I have ever heard of travelers here. We are starting to get creative with scheduling (looking at 12 hour shift models) and using more lab assistants to do work that does not require the MLT (or lab scientist in the US). Even in the blood bank. People are tired and burnt out.
  9. Hi everyone! I'm looking for anyone with experience using the Bio-Rad or Diamed antigen typing cards. We are validating (verification) their use in our lab. We are using the Rh/Kell card, and single antigen cards for Jka, Jkb, Fya, Fyb, S and s. So far we love them for manual column agglutination testing. We also have the Bio-Rad IH-500. I guess I'm looking for any experience using the cards an the automated platform. Pros/cons. What you use for QC and how it is programmed. Any other feedback. Anything you have is greatly appreciated! sandra (This will also be posted on the Blood Bank Professionals group on Facebook, so please excluse the multiple posts )
  10. I agree with Ensis01. Sometimes gel can give a false positive - if there is a problem with the card/well or sample (bit of fibrin, etc) - which is resolved upon repeat and/or tube testing. Otherwise, yeah, if you can explain it = great, group specific (meeting all other policy, of course) If not = O. sandra
  11. Sorry - just saw this reply now. Canadian Blood Services tests all donor units for K. If K negative, the donor end label has K- on it. If K positive, the end label doesn't have any K antigen testing information listed - the K+ status is only embedded in the donor unit phenotype barcode. All donor units are treated the same - so the K+ units are available, as all other units are, but it is easy to select a K- unit for females of childbearing potential and who are on a drug like daratumumab. sandra
  12. Hey Nikki, You planning on an IH-1000 or two IH-500? Or a combination with or without the SAXO? sandra
  13. We require any request for blood component or blood product pickup to have the following: Patient Name Patient MRN (i.e. hospital number) Type of product required Location (for phone requests to send via pneumatic tube system) A sticker or other official paper is not required. The info can even be handwritten - but all is required and it has to be correct. sandra
  14. Same. We don't select K negative units once off the dara. Here in Canada, all our units are K typed by Canadian Blood Services. It wouldn't necessarily be more work to select K neg units, but we feel it isn't needed.
  15. We share an LIS with a couple of other hospitals. We allow those previous blood groups to be the confirmation sample. We have access to a validated computer system that pulls results from other hospital LIS systems and matches using a unique ID (here in Ontario Canada it is the OHIP number). Names/DOB are double checked. We allow those for the second blood group as well. All our labs are accredited. I'm not sure what the difference between using the other hospital result in the LIS vs the other validated computer system would be. Also... my experience has been that blood testing done recently is MUCH more reliable than testing from many years ago - even if in the same hospital. Use of automation and positive patient ID systems has greatly reduced the risk of error. sandra
  16. In Canada, all blood components and blood products (derivatives) require informed consent.
  17. Here in Canada, the same sample can be retested IF the sample was collected using positive patient identification. So, here in our lab, we are super lucky because we have MLA who perform phlebotomy on 95% of the patients (some are nurse collected in the ED and ICU). Our MLA use positive patient identification technology (Mobilab). We allow for the retesting of those samples. Anyone else needs a new sample - which we order for lab collection, thus avoiding the workarounds where a second sample is drawn at the same time as the first but tucked away until needed. We also allow the previous ABO to be from another lab. We have access to blood bank results from area hospitals. If the blood group from our hospital matches another hospital we don't need the restest. sandra
  18. I'm so jealous - all these labs where the KB is performed in hematology! Here ours are done in the BB. To be fair, we are a core lab, so the staff performing the test are the same. I would just rather not own the test and all the competency that comes with it
  19. We have been using a Bio-Rad IH-500 (and a SAXO as backup) for about a year now. Very happy with them. They work with the IH-Com that serves as a command centre for both and as middleware. We also use the antibody software and upload our QC to Unity. (We were manual gel/tube prior). sandra
  20. Thanks Ensis01! I haven't even gotten that far yet - so I didn't know that the concentration of DTT is different :) s
  21. When I was trained (many years ago!) we used +/- for microscopic tube reactions. Now, I encourage MLT to only use the microscope if they are looking to verify a mixed field or rouleaux. I suppose there could be other rare times to use a microscope - like an anti-Sda? But generally - no microscope. But they love the microscope...
  22. I'm also developing a procedure for DTT treated cells. Can I assume that the DTT treated cells can be used to help ID antibodies other than daratumumab without additional validation? I am intrigued by the use of DTT for differentiation between IgG and IgM antibodies but I don't think we will go forward with it here at our community hospital
  23. We do the same as Nikki. If an eluate is needed, an acid elution is done. No LUI freeze on the menu. sandra
  24. Nikki - my calendar says it is 4am to 5:30am here! I hope it is available as a recording! sandra
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