Auntie-D
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Posts posted by Auntie-D
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Age? I'm 60 and my anti-B is as strong as an ox!
Macolm I'm sorry
I'm not normally ageist honest I'll soon be over the hill myself - Malcolm Needs and Sandy L
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It's not unusual for them to disappear with age
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In the UK blood products must have a dedicated fridge. Reagents may be kept in the same fridge as samples but samples must always be stored lower.
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Does freezing it not kill the bacteria?
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My novice interpretation of this article:
Their test was to see if they could identify parasites in the buffy coat that they didn't see in a thick film, in patients they thought it should be possible due to previous thick film identifications.
And yes, they did in ~27% of cases.
Their recommendation was for add'l studies due to low sample size but that it might be a useful tool to consider to prevent false negatives.
The initial slides were positive and the susequent slides were negative. What I was trying to say was neither method should be used to determine efficacy of treatment due to the latent phase of treatment. And it certainly shouldn't be used as a primary diagnostic tool.
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It state
Apparently the use of a buffy coat smear to detect malaria came from a 2011 study that was published in the Asian Pacific Journal of Tropical Biomedicine. I does state however that a larger and more complete study should be performed before implementing this into common practice.
Here is the link if you’re interested. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609286/
It states that there are 36 examined that have been detected by conventional thick films already and the patient is already undergoing treatment. The buffy coat ones are taken after treatment - so assumedly only to detect whether the treatment is complete?
I think it is risky to use any form of films to determine completeness of the treatment as it may be that the malaria has gone into hepatic cells and is no longer circulating.
It definitely shouldn't be used as a primary diagnostic tool IMO.
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I did one on the importance of recognising delta check failures in other departments and the implications in transfusion - and why they should let us know. Overtransfusion and the risk of TOCO in dilutional samples and the risk of ABO incompatibility if WBIT.
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How old is she?
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I'm not sure if this is the sort of thing you are after but this is the one from the the National Blood Service in England
It is quite 'humorous'
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We use MI and FI and the mother's forname. Once the baby has been given a name we change the record and put the mum's name, DOB and hospital number in the patient notes file for the baby so they can always be tied back.
My baby would be FI of Dee, AUNTIE, 5/8/15
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It makes every sense to start with Rh pos for a massive haemorrhage patient - can I get my place of work to adopt this policy? Grrrr no! And we only hold 8 O negs so once you use 2 massive haemorrhage packs you are switching to O Pos anyway so why 'waste' the O negs - why not just start with O Pos?
But then there's the ones where they initiate the MHP and only use 2 units...
- David Saikin and Eoin
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We had a patient just last week with a known anti-D showing no reaction at all in the antibody screen on this occasion - she was panelled with enzyme and showed 3+ reaction. I didn't even know this could happen! I though anti-D and anti-K stayed detectable for life...
Could it be that she was like this lady and the new pregancy has elicited an immune response bringing titres to a detectable level again?
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I have seen places that freeze preops and save for up to a month. It all depends on your Medical Director's comfort level and the pressure exerted by the Medical staff.
Somewhere I used to work did this - aliquoted off the plasma and froze it. I was never comfortable with it personally due to the risk of someone aliquotting more than one sample at a time when rushing (ie prelabelling tubes). I shouldn't happen but it did...
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Ha ha I can just imagine handing over the Kleihauers - it's hard enough to get someone to sit down and do a manual diff on a myeloperoxidae patient (we have Advia 2120s sadly...)
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Malcolm as mentioned above I have seen had a moderately severe HDN due to IgG Anti-M - never written up as the lab wasn't very proactive in stuff like that. I've left now and work in a lab that is much more 'current'
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No, that is most definitely NOT true Auntie-D.
Many cold-reacting anti-M's are IgG in nature, and even if you can detect an anti-M in a tube technique, it is not necessarily clinically significant unless it can be detected at strictly 37oC (see Daniels G, Poole J, de Silva M, Callaghan T, MacLennan T, Smith N. The clinical significance of blood group antibodies. Transfusion Medicine 2002; 12: 287-295).
But if it is detectable in gel, and causes HDN in that particular patient, you can't just discount it because it doesn't react in tube.
In vivo always wins over in vitro of course
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We have seen immune, IgG anti-M formation as well, most recently 2 days ago, but the issue in this post is that the antibody is undetectable in tube. Were it positive in tube as well, it would have to be considered clinically significant, but since it is only detectable in solid phase (or gel, in my case) I don't believe it can be considered significant.
Surely it is considered clinically significant if it reacts in the patient, regardless of whether or not it reacts in tube
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neonatal deaths due to anti-M!!!!
I've seen a moderately severe HDN due to IgG Anti-M so it is possible that in an area with less than perfect antenatal care (or non-compliant patients) a baby could be severely affected.
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What make and lot number? What are your storage conditions day to day and for stock?
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I'm not normally ageist honest 
Transport Cooler
in Equipment
Posted
I personally don't like the idea of ice on the units - freezer packs are cooled to the temperature required, not below. Applying ice directly to the blood risks the integrity of the red cells