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jmm8427

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Posts posted by jmm8427

    Alpha-chymotrypsin, Trypsin and Pronase

    in Transfusion Services

    Posted

    It sounds like if at that point (after throwing everything at it including DTT and papain) you don't have the rare antisera/cells and reagents to identify the antibody then you are going to have to send it out anyways and the reference lab will probably end up repeating most of that testing in order to ID the reactivity on their end.  You are right, you have to consider that implementing trypsin is a big project with SOP revisions, may involve added costs, is known to have stability issues, and how often do you expect you will really need it and keep your staff competent?  Will it really have any added benefit to your testing?  

    From personal experience in a smaller reference lab with limited rare reagents as well as in a larger IRL, I would say it sounds like you may want to wait on the trypsin until you have a better inventory of rare reagents to aid in completing antibody ID?   

    IRL Manager - Lexington, KY USA

    in Job Postings

    Posted

    Kentucky Blood Center is looking for a Reference Laboratory Manager for their Immunohematology Reference Laboratory (AABB IRL), see below and this link: http://kybloodcenter.org/about-us/careers/

    Successful candidate will be a proactive professional responsible for the oversight and management of the reference laboratory (AABB IRL). Responsibilities will include the development of short- and long-term plans; budget preparation and monitoring; oversight of compliance with staff training, processes, and procedures; workload of reference laboratory including staff supervision, employee evaluation, and other standard management functions. 

    Qualified applicants must have a four-year degree, MT(ASCP)SBB, with a minimum of three years management experience preferred. Proof of education/certifications required during the interview process. Must have a working knowledge of industry regulations including FDA, AABB Standards for Immunohematology Reference Laboratories and AABB Standards for Blood Banks and Transfusion Services.

    Must be proficient with MS Office products; have proven data analysis skills; be highly organized, reliable, and have outstanding interpersonal skills. Strong written and oral communication skills, a do-what-it-takes work ethic, and a team player attitude are required.
     

    Benefits: Health/Dental/Vision/Life/Short Term Disability/Long Term Disability/Cancer Insurance/Accident Insurance/Flexible Spending Accounts/Health Savings Accounts/Paid Time Off/Paid Holidays/Employee Assistance Program/403(b) Retirement Savings Plan/Pension Plan

    Case Study

    in Case Studies

    Posted

    Thank you both!  No, we didn't crossmatch any units but I will see what that does.  And I didn't think of Pr! Out of the lab today but I'll give it a second look, thank you!

    Case Study

    in Case Studies

    Posted

    We have a troublesome patient that came in again, history of warm auto a few years ago, cold autoantibody and an antibody of undetermined (reacts majority of cells).  

     

    What do you think: majority of cells reacting in gel (about 1-2 were negative; strength of reactions 1-2+) with positive autocontrol, No reactivity with PeG except autocontrol is 1+, 1-2+ reactions at room temp and 3-4+ reactions at 4C.  A cold auto-I was identified based on the 4C/RT reactivity and cord testing.  Ficin testing panel was negative (including autocontrol), DTT panel was reactive still.  Neutralization testing was performed but cells were still reactive.  A cold autoadsorption didn't work.  A RESt adsorption was performed but still had partial reactivity.  The plasma reactivity was able to be adsorbed out this time with allogeneic cells.  The DAT was positive, no transfusion in last 3 months.  The eluate was non-reactive.  EGA treated cells were negative when tested with the plasma/PeG,

     

    Do you think the reactivity is due to autoantibody or is it an alloantibody?    We don't think the warm auto is back but we weren't quite sure to make of all the gel reactivity and weren't quite sure what to do next, if anything.  Thoughts?

     

    Thanks for the help!  

     

     

    Sickle cell patient policies

    in Transfusion Services

    Posted

    We recommend matching the Rh system (CcEe) and K, hemoglobin S negative units; matching Duffy, Kidd, MNS system only if they make an antibody.  (If it becomes too difficult to match exactly we do the best we can, we also attempt to molecular type them and confirm if the patient has the GATA mutation) .  We're a reference lab so many of our hospitals have different policies.  Some just give hemoglobin S negative, a few give C-E-K-HgbS- unless the Rh system is different and a few follow the same policy we have.  The information we've looked at indicates that there is no uniform policy among institutions regarding transfusion of these patients but it is nice to hear what others are doing.

    SBB Programs

    in Education / Quality

    Posted

    I think most accredited SBB programs are probably great, it depends on you. Do you work better with an online setting and can motivate yourself to study or seek out the information you need?  Or do you need to attend the class and get the interaction with the other students/teacher?

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