We have a troublesome patient that came in again, history of warm auto a few years ago, cold autoantibody and an antibody of undetermined (reacts majority of cells). What do you think: majority of cells reacting in gel (about 1-2 were negative; strength of reactions 1-2+) with positive autocontrol, No reactivity with PeG except autocontrol is 1+, 1-2+ reactions at room temp and 3-4+ reactions at 4C. A cold auto-I was identified based on the 4C/RT reactivity and cord testing. Ficin testing panel was negative (including autocontrol), DTT panel was reactive still. Neutralization testing was performed but cells were still reactive. A cold autoadsorption didn't work. A RESt adsorption was performed but still had partial reactivity. The plasma reactivity was able to be adsorbed out this time with allogeneic cells. The DAT was positive, no transfusion in last 3 months. The eluate was non-reactive. EGA treated cells were negative when tested with the plasma/PeG, Do you think the reactivity is due to autoantibody or is it an alloantibody? We don't think the warm auto is back but we weren't quite sure to make of all the gel reactivity and weren't quite sure what to do next, if anything. Thoughts? Thanks for the help!