If you are right with the auto-B Ab. AB cell shoud give the positive result with the plasma (Am I right?).If you are right with the AB blood group genotipicaly, This group may be cis AB with anti-B (if anti-B's rxn is 1+ (reverse grouping) )
Reverse should be *incubated at 37 C for IgG/IgM dosage effect (or incubated at 4 C for 30 min. you had done), *checked for age Newborn or elder >60 years old, *agammaglobulinemia (check for gamma globulin level by ELISA) *may be using immunesupressive drugs...
take 500 microliter LISS + 25 microliter erytrociyte... reconstitute and pipet 12,5 microliters to each microtubes. then centrifuge 10 min. finally evaluate... that is all
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