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Interesting responses.... I've seen it done both ways as well. My reference lab experience has been to go directly to the panel while my hospital experience has been to perform the screens first.

I am doing a bit of research on KB stains and I was wondering what is the current practice. In the past I've worked at facilities where they were performed in-house (by BB or another department) and also where they were sent out. What are most hospital labs doing currently? Primary benefit of referral seem to be that you do not have to maintain competency, which can be difficult when the test is performed infrequently. I see TAT as the main drawback of referral, considering the recommendation to administer RhIg within 72 hours of delivery. Any other thoughts on referral vs. in-house testing? Anyone using KB as first test for routine FMH, i.e. not performing a "rosette" test fetal screen?

Congrats!!

Very good article!

Please stop by the booth while you are there. It would be great to meet all of you. Also, while you are there, ask about our new FREE mobile app for blood banking. Safe travels, Jimmy Lowery Operations Manager Hemo bioscience 2011 AABB CTTXPO Booth #1701

Hi Mabel, As I understand it, the 4 drop method is required when normal ionic strength PEG solutions (i.e. 20% PEG in PBS) are used. Since the Hemo bioscience PEG reagent is Low Ionic Strength, only two drops are required for antibody screen/identification testing procedures. Do not hesitate to contact me should you have any questions or require further information. Thanks, Jimmy Lowery, MT(ASCP) Operations Manager Hemo bioscience j.lowery@hemobioscience.com 1-866-332-2835

Hi mollyredone, You are most welcome. I hope you find the chart helpful.

Hello, As David mentioned, Hemo bioscience does have a current antibody chart available. To receive a copy, send your request with shipping address to info@hemobioscience.com Jimmy Lowery, MT(ASCP) Operations Manager Hemo bioscience

Thanks for the information !!

Thanks for your interest in our Phosphate Buffered Saline Concentrate. QC would depend on the individual laboratory and the procedures in place. At a minimum, we recommend a pH measurement. You could also measure the osmolarity if you have access to an osmometer. Please let me know if you have any other questions or require further information.

As David Saikin stated, "Making C activated cells is too spurious and they are notoriously unstable. " For those of you who are making your own C3 control cells successfully, I am curious as to which methods are being used.

I have noticed in a recent thread that there has been discussion about the need for Buffered Saline in small quantities. The company that I work for, Hemo bioscience, offers a phosphate buffered saline concentrate. Our PBS concentrate is a 25X liquid concentrate designed specifically for blood banking with 4 year expiry dating and stored at room temperature. You reconstitute it with DI water from your lab and it is ready to use with pH of 7.00 +/- 0.05 and osmolarity of 290 +/- 5. Also, the package insert indicates that the reconstituted product has a 4 month shelf life. This product has a Green advantage- low cost of shipping, less fuel used in transport. Also if you currently buy and store bulk saline, this concentrate takes up 25X less space. It is available in 4 sizes: 40 mL (1L PBS) 200 mL (5L PBS) 400 mL (10L PBS) 800 mL (20L PBS) You can make up as much or as little as you need at a time, the remainder of the concentrate will maintain the original shelf life. For example, if your lab purchased the 200mL size and made it up 1L at a time, the remainder of the concentrate would maintain the original 4 year expiry dating. If you are interested, I can send you more information on this and our other blood bank reagents. My email is j.lowery@hemobioscience.com Thanks, Jimmy Lowery Technical Sales Manager Hemo bioscience

I was wondering if those of you who use the various blood bank automation platforms might be able to answer these basic sample specification questions for each platform. 1. Tube size (we do not use pediatric tubes so that is of no concern) 2. Minimum sample volume for plasma/serum, RBCs and for antibody ID I remember reading somewhere that for the Echo, these are: Tube Size: 12-16mm diameter; 75-100 Length Min. sample volumes: 500 µl plasma 250 µl RBCs 750 µl for antibody ID I would appreciate confirmation of those numbers as well as similar information for the Galileo, ProVue and Tango. Thanks in advance for any assistance !!