If we get a positive antibody screen on Echo and an ID that is non-specific/indeterminate we run another screen with PeG/tube. If that screen is negative with a negative auto or negative tube DAT we report the antibody screen as Positive with 'solid phase reactive antibodyof undetermined specificity'. If we have a positive DAT on Echo or with tube with the scenario above, and all screen cells are positive on Echo, we would call it a warm auto. If a crossmatch is ordered in a case like this, we would do an IS and tube AHG/PeG crossmatch. If the IS and AHG/PeG crossmatches are compatible, we would release the blood for transfusion with the comment that the unit is compatible in AHG with PeG. If the IS crossmatch is incompatible, then we would look for rouleaux or cold reactive antibody to explain the IS results. If the problem is a cold (P1, M, N, etc) that is not clinically significant (and is AHG compatible) we would not screen for antigen negative units. (Please note that we do not do cardiac surgery here.) If the PeG screen is positive, then we are going to do more of a workup, such as an ID panel with PeG, a repeat screen with LISS, elution if indicated by patient history, etc. We may still end up calling it a warm auto or antibody of undetermined specificity, but we've worked it up more fully before we do that. If we are still not satisfied with what we are seeing, especially if the patient has a warm auto that we can't minimize with PeG or LISS, then we send it out to our reference lab. We used gel for almost 9 years and we had similar looking antibody situations with that and that's where this approach came from. We have never had a physician question or even ask about the reports on these patients. If we feel that the antibody is significant (warm auto), the physician decides whether or not he/she wants to continue with the transfusion. I've had discussions with the reference techs about these kinds of reactions and their feeling, based on experience and discussions with other reference labs, is that we are probably picking up autoantibodies that are more enhanced by solid phase than our tube methods, HLA antibodies, drug related antibodies (which we don't see if the patient doesn't have that drug in question in their system) or antibodies directed against preservatives or other chemicals in our test system. These types of antibodies are a pain in the posterior for us but probably not significant in terms of transfusing the patient, though I'm not going to report that. The patients we see with these problems are usually Onc, cardiology, and frequent flyers with multiple serious health issues. They've been multiply transfused, multiply drugged and their immune systems are not like yours or mine, so it's hardly surprising that they cause us problems. Bottom line - if we're not comfortable with the what we see with repeat screens or IDs in tube, we send it out. But we don't have to do it too often.