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But how do you REALLY feel Malcolm?! LOL......just giving you a hard time. Brenda Hutson

I followed up with Immucor and was told they will have the answers webinar up in the next week or so.

I saw the "syalic" acid typo, but ain't got no grammar nazi in my blood.. ^^ Living in the lower end of the food chain in our lab, as I am right now, I can't call the shots. And suggesting far off procedures are quite taboo in these kind of people. (As you may now realize, I'm not a Saudi.) I would loveto see one day that these practices of yours becomes a procedure here, or eventually I get to work with an institution which is following one. Thanks. Yes sir. My question was: what made these neutral cards so special that it is acceptable to omit the AHG?

Because that is what they are, neutral - I call them buffered gel. There are no additives, only gel. If you want antiglobulin reactions you have to use anti-IgG or polyahg cards. Those neutral gel cards are just like the portion of your ABD Reverse card that does the back type . . . plasma and cells. You can use them for room temp studies or add your own antisera to cells for ag typing.

Don't know what you mean Mabel:sarcasm:!!!!!!!!!!!!!! I would go with testing the father against the mother's plasma/serum first. Even if the mother and father are ABO incompatible, you could always use blood group substance to take out the mother's ABO antibodies or, better still, as this may dilute out such a weak antibody, adsorb the mother's antibody onto the screening cell, and then elute it. This will leave the putative low-incidence antibody free of maternal ABO antibodies. If then the father's red cells react against the isolated antibody, then I would pull out all the plugs to identify the specificity. As you say, this antibody could have been formed in the previous pregnancy (or in this pregnancy) and could get stronger (and more clinically significant) during the pregnancy. Identification of any such antibody is a pain, but identification may help predict if it has the potential to cause haemolytic disease It could be one that is known to adsorb onto the apical surface of the placenta, such as an antibody in the Cromer Blood Group System, in which case there should be no more worries, but it could equally be one that has been known to cause problems in the past. If dad doesn't react with the isolated antibody, then, hey, life's a dream and I wouldn't take it any further. I'm always more worried about potential HDNF in these cases than transfusion. Hope this helps (even if I'm not pregnant myself)!!!!!!!!!!!!!!!!! :D:D:D:D