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How are you transfusing cryo to a neonate?. Issuing single cryo unit with filter? Pulling a syringe through a 150u filter starting with a 5 unit pool? Thanks for any information!

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Certainly in the case of an anti-Vel, it can be vital to use serum, rather than plasma, as it can frequently only be detected by using an AHG that detects complement. I DO SO AGREE WITH YOUR FINAL SENTENCE.

I was taught, many, many moons ago, that prewarming should only be applied when the identity/specificity of the antibody is known/understood, i.e., you know what you're trying to avoid. Cold autos are probably the most commonly seen, but "nuisance" cold-reactive antibodies like anti-M, anti-P1, anti-Lea/Leb can also pop up and potentially be avoided using a prewarm version of an assay. These specificities are usually IgM class, are amenable to prewarming, and are generally considered clinically insignificant. Prewarming to "get around them" is often a good option. However, a cautionary note: There are cold-reactive antibodies that can present in a similar fashion that are clinically important - anti-Vel , anti-PP1Pk, for example. It can be dangerous to use prewarming to avoid these sometimes potent and potentially life-threatening alloantibodies. Prewarming may be a very useful tool, but as is true for very specialized tools, it should only be used and applied by trained and experienced operators who understand its strengths and weaknesses.

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we can only use it with tests that require the addition of anti-IgG - such as an antibody screen or full XM. we are not allowed to use it for IS testing such as reverse testing or ISXM. so - in my mind, I'd say it could be used when a patient has a cold-auto ab and you want to see if there are any underlying clin.sig ab's after the addition of anti-IgG if our reverse and/or ISXM are positive due to the presence of cold auto - we have to cold adsorb and retest with absorbed plasma.

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Welcome to this fantastic site jnk00. ENJOY!

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Oxygen transport Oxygen transport Submitter Cliff Category HemeLabTalk Submitted 05/03/2024  

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Hello jnk00, Welcome to PathLabTalk. Please feel free to browse around and get to know the others. If you have any questions, please don't hesitate to ask. jnk00 joined on the 05/03/2024. View Member

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