NAN47 Posted May 16, 2016 Share Posted May 16, 2016 Hi, does anyone use a NIBSC anti-D on their blood bank automation...to demonstrate metrological traecability...? Thanks Link to comment Share on other sites More sharing options...
Malcolm Needs ★ Posted May 17, 2016 Share Posted May 17, 2016 We do when we are using our quantification automation, but we don't on our grouping machine. Link to comment Share on other sites More sharing options...
A little lab lost Posted June 15, 2016 Share Posted June 15, 2016 I am considering using a NIBSC anti-D for a comparison study between the automation and manual testing. I'm unsure how often this should be done to comply with ISO 15189. Link to comment Share on other sites More sharing options...
gagpinks Posted July 9, 2016 Share Posted July 9, 2016 On 15/06/2016 at 9:42 PM, A little lab lost said: I am considering using a NIBSC anti-D for a comparison study between the automation and manual testing. I'm unsure how often this should be done to comply with ISO 15189. We also got NC for not using NIBSC for comparison in our recent UKAS inspection. As far as I know they recommend twice a year. Link to comment Share on other sites More sharing options...
A little lab lost Posted July 13, 2016 Share Posted July 13, 2016 Thanks, I think that has convinced me to order some. I was hoping to get away with the NBS weak anti-D as it's pre made and we are about to start using it as the panel control. Now I've just got to write the validation document! Link to comment Share on other sites More sharing options...
galvania Posted July 19, 2016 Share Posted July 19, 2016 I am going to have a rant here!! I think using this type of reagent for routine blood banking (as opposed to quantification techniques) is WAY over the top. Metrologicals are all very relevant in chemistry where you are looking for a number but totally meaningless in most areas of immunohaematology. With the exception mentioned by Malcolm, IH is NOT a quantitative technique. So just what are you trying to prove with this expensive and precious material ? You want to compare that your instrument is getting the same results as your manual technique? So use a series of weak antibodies. The actual strength does not really matter, does it? What you want to know is - for the same cells and the same technique, did my instrument detect the same antibodies, and was the reaction strength the same? Rant over! Malcolm Needs 1 Link to comment Share on other sites More sharing options...
Malcolm Needs ★ Posted July 19, 2016 Share Posted July 19, 2016 On 09/07/2016 at 10:42 AM, gagpinks said: We also got NC for not using NIBSC for comparison in our recent UKAS inspection. As far as I know they recommend twice a year. Rant totally justified Anna - and gagpinks, I should have suggested to you well before now to challenge your UKAS Inspector to show where (anywhere) NIBCS reagents are mentioned in ISO15189. I presume that you are aware that the weak anti-D reagent produced by NHSBT has a specific concentration of anti-D (although I am at home at the moment, and cannot, for the life of me, remember what is this concentration, but I know that it is very low, as it was lowered a few years back, to reflect the fact that everyone was getting "too good" at detecting the the slightly higher concentration of anti-D, as a result of using more sensitive techniques, such as column agglutination technology, and, not to be forgotten, a general increase in the ability of Biomedical Scientists to actually detect antibodies!). As I said above, we do NOT routinely use NIBSC reagents in RCI in NHSBT, except for quantification, where we are looking at a concentration of antibody, and all of our eight RCI laboratories have just been inspected by UKAS. We did not get an NC for this. I would suggest that your particular UKAS inspector was, shall we say, a little over-enthusiastic! Were they actually a Blood Transfusion person themselves (either now or retired)? gagpinks 1 Link to comment Share on other sites More sharing options...
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