galvania Posted September 15, 2011 Share Posted September 15, 2011 Dear allwe are trying to do some comparisons and I need some informationCan you please tell me :1. What is the maximum age of a patient blood sample that you would use for performing a) a blood group an antibody screenc) a DATd) an extended phenotypee) an identification2. How long after testing do you keep your sample; in what form (whole blood, plasma/serum) and at what temperature and why?I know that's a lot, but this information will be really helpfulThanksAnna Link to comment Share on other sites More sharing options...
Deny Morlino Posted September 15, 2011 Share Posted September 15, 2011 1. a) Three days three daysc) up to 14 days for a pretransfusion sample in a reaction workup.d) not performed at our facilitye) not performed at our facility2. We maintain our samples for 14 days after testing separated into red cells and plasma (a bit old school but being examined), refrigerated. The time line is to be sure we cover the AABB standard: 5.11.4 Retention of Blood SamplesPatient samples and a segment from any red-cell-containing componentshall be stored at refrigerated temperatures for at least 7 daysafter transfusion.14 days ensures we have complied and makes it easier to discard specimens. Link to comment Share on other sites More sharing options...
David Saikin Posted September 16, 2011 Share Posted September 16, 2011 1, a,b,c: 3 days1.d,e: 7 days2. a minimum of 7 days maybe up to 14 depends on day of transfusion. Plasma/cells separated and kept at 4C . . . as per Standards. Link to comment Share on other sites More sharing options...
galvania Posted September 16, 2011 Author Share Posted September 16, 2011 Me againCan you tell me how you stop your patient red cells from haemolysing after you have separated them, or do you never notice because you never use them!Anna Link to comment Share on other sites More sharing options...
Deny Morlino Posted September 16, 2011 Share Posted September 16, 2011 Truely do not use them so had not really noticed any degree of hemolysis!! Link to comment Share on other sites More sharing options...
LaraT23 Posted September 23, 2011 Share Posted September 23, 2011 a blood group = 10 days an antibody screen = 24 hoursc) a DAT = 10 days - for pretransfusiond) an extended phenotype = 10 dayse) an identification = 24 hoursWe keep them all refrigerated, not room temp. We do not separate the cells and plasma. Link to comment Share on other sites More sharing options...
Auntie-D Posted September 29, 2011 Share Posted September 29, 2011 On whole bloodBlood group - 7 daysAntibody screen - 7 daysDCT - 24 hoursSample are fridged at 2-8oCIt used to be that if the patient is preop and has no transfusion before the op a group and screen is done and the plasma frozen at -30 for xm immediately pre or during surgery. The sample was valid for up to 30 days. I removed this as IMO it eliminates the second check that you get from an admission sample. We now get a sample at the preop clinic (up to 30 days before surgery) and immediately on admission. This allows for 2 separate groups with elective surgery reducing error.The 30 day 'rule' for antibody screens is still acceptable though in the UK according to the MHRA and BBT. Link to comment Share on other sites More sharing options...
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