Lindz82 Posted November 1, 2010 Share Posted November 1, 2010 Okay I am wondering what types of ernzymes everyone uses... we currently use Ficin/ Papain, and are looking at some others... What are your thoughts? Link to comment Share on other sites More sharing options...
David Saikin Posted November 2, 2010 Share Posted November 2, 2010 As a small hospital reference lab I only use ficin, but I do have WARM, which I use it off label. Link to comment Share on other sites More sharing options...
AMcCord Posted November 2, 2010 Share Posted November 2, 2010 Like David, I use ficin and WARM. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted November 3, 2010 Share Posted November 3, 2010 This may not be a lot of use to you if you cannot get hold of the book, or you want to know a bit more detail, BUT...If you look on page 521 of "The Blood Group Antigen FatsBook" (should be in ALL blood transfusion laboratories - it's brilliant), by Marion Reid and Christine Lomas-Francis, 2nd edition, 2004, Elsevier Academic Press, there are all sorts of facts about the different enzymes used in blood transfusion.That having been said, many of the different enzymes are only regularly used in very specialised laboratories, so be aware that you may be making a rod for your own back, as all will need to be validated, quality controlled, ...you name it!:ohmygod::ohmygod: Link to comment Share on other sites More sharing options...
Lindz82 Posted November 8, 2010 Author Share Posted November 8, 2010 Thanks! Link to comment Share on other sites More sharing options...
bxcall1 Posted November 18, 2010 Share Posted November 18, 2010 Besides Immucor, where can you purchase papain or ficin? Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted November 18, 2010 Share Posted November 18, 2010 I put "papain ficin suppliers" into my search engine and got 9060 hits, so there must be others out there.I must admit that I did NOT look at every one of those 9060 sites!!!!!!!!!!!!!!!!!!!!!! Link to comment Share on other sites More sharing options...
Lindz82 Posted June 13, 2012 Author Share Posted June 13, 2012 We order from Sigma Aldrich. Link to comment Share on other sites More sharing options...
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