Quotient BioDiagnostics

[dawn w 11]

Hey Folks,

For any of you looking for a reagent alternative, Quotient BioDiagnostics reagents are fabulous.

We performed extensive studies and found them to perform at or above our current supplier.

For information you can email me at dwebber@carterbloodcare.org

Edited March 17, 2010 by dawn w

[LaraT23 96]

Hi Dawn,

The only issue I have found when evaluating those Quotient reagents is that we may potentially have patients whose RH histories may change. The Quotient alpha ( blend) tends to pick up D variants and so called "weak" D's on immediate spin. I am working on a recommendation about how to handle these patients so until we get that nailed down we are sticking with Ortho. That and my fridge is full of stock!

[irshadaad 12]

whats the composition of modified LISS

[Mabel Adams 1,044]

So has anyone actually found previously D neg patients (with other reagents) come up as D pos with Quotient anti-D?

[David Saikin 1,280]

I have played with the QB anti-D's (alpha, delta, and blend). Serendipitously (?) I had 4 pts and an employee with bizarre D typings in gel/tube (both Ortho). The blend will detect DVI at immediate spin. The alpha/delta are IgM so don't use them for weak D testing. I expect to swtich vendors when my current stock is utilized. If you have the resources, QB also has a D typing kit for evaluating anomalous D types.

[Mabel Adams 1,044]

In testing our Quotient anti-D (blend) on Rh neg cord samples we are finding what looks like weakly positive reactions. The technical rep says this is due to the higer level of potentiators and its tendency to bring up false positives with the contaminants in cord samples (Wharton's jelly and whatnot). She said to wash the samples better. We are still testing this out. What is everyone else's experience with their anti-D blend on Rh neg (with Immucor) cord samples?

[Mabel Adams 1,044]

We have been using this anti-D now for a year. A short learning curve for everyone to get comfortable with what a "real" positive looks like is all it took.

[David Saikin 1,280]

They have an antibody panel available now also . . .

[Mabel Adams 1,044]

and PEG and LISS that are quite a bit cheaper than we were paying Immucor since we don't buy much else from them.