Transfusion reaction--Culture

[Eagle Eye]

At what temperature(s) do you incubate your culture for transfusion reaction workup??

[rcurrie]

30 C and 37 C.

BC

[Yanxia]

In China we will do 4 ,22 and 37 degree C incubation with and without oxygen respectively.

[Mabel Adams]

We do what the microbiologists tell us is necessary to make bugs grow--basically 37. Their system can pick up Yersinia fine. The logic of testing at the temperatures that blood products are stored draws blank stares from microbiologists. It would be like trying to grow all cultures from skin infections at 33 degrees because skin is cooler than body temp and that is where the infection is.

[jhaig]

37C and room temp (20-24C) for Yersinia

[Yanxia]

Please permit me to explain it . In China we do 4 degree C incubation because some germ can grows in 4 and produces toxin which is harmful when the blood is transfused to a patient. The germ maybe not grow in 37 degree C ,but the toxin is here.

[Mabel Adams]

Is the germ you look for at 4 degrees Yersinia, or something else?

[Yanxia]

We detect Algoriphagus，I don't know whether this name is right, it is a kind of germ which can grow in 4 degree C , it like cool environment.

If we don't incubate the blood in 4 degree C, mybe we can't detect it .In China this is our SOP, I don't know if the germ infection between differnt group of people is not the same.