Learning from the experts 5 Posted April 30, 2019 Share Posted April 30, 2019 For an A3B patient, is it common to get MF with anti-A reagent only and no MF with anti-A, B? We tested the sample with multiple sources of Anti-A, Anti-B and Anti-A, B. MF is seen only with -A reagent. Link to post Share on other sites
yan xia 193 Posted May 1, 2019 Share Posted May 1, 2019 (edited) Because of the normal B antigens on the cells, so we can see strong reaction on MF with anti-AB. Edited May 1, 2019 by yan xia Learning from the experts and galvania 2 Link to post Share on other sites
noelrbrown 33 Posted May 1, 2019 Share Posted May 1, 2019 Be aware that most Anti AB these days is a blend of anti A and Anti B monoclonal and is not strictly an anti AB. Learning from the experts and AMcCord 2 Link to post Share on other sites
Malcolm Needs ★ 4,878 Posted May 1, 2019 Share Posted May 1, 2019 Don't forget, also, that an individual who is AB has the A and B antigens on each and every erythrocyte. The antigens themselves are NOT direct gene product, as they are, in effect, immunodominant sugar residues, and only proteins can be direct gene products. In the case of the A and B genes, the direct gene products are, respectively, N-acetyl-D-galactosaminyl transferase and D-galacosyl transferase, both of which transfer their respective sugars from a UDP donor molecule. However, these two enzymes are competitive. As a result, sometimes the A transferase "wins the battle" between the two, and the A antigen ends up being expressed more strongly on the red cells than the B antigen, and sometimes the B transferase "wins the battle" between the two, and the B antigen ends up being expressed more strongly on the red cells than the A antigen. In the case of the latter, an individual who is genetically A2B can, phenotypically, appear to be A3B, and so genotyping the individual may not help. All that having been said, the fact that (roughly speaking) 50% of the antigens expressed on each red cell will be a normal B antigen, it is not surprising that there would be no"mixed-field" reaction with anti-A,B. Learning from the experts, AMcCord, yan xia and 1 other 4 Link to post Share on other sites
Learning from the experts 5 Posted May 2, 2019 Author Share Posted May 2, 2019 Thank you, guys, for explaining. Malcolm Needs and yan xia 1 1 Link to post Share on other sites
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now