We have just implemented our Echo last week. What I have observed with D teating on the Echo is the Series 4 will be negative and the Series 5 positive. I wonder if this is an indication of Partial D. We use Series 4 in our tube testing through weak D to confirm, but interpret on an individual basis (calling the Patient D neg and the Donor D pos). The Echo REALLY likes to find RhIg. Even if we repeat a screen or panel with PEG, the tube method is neg; we report the ab screen as neg in these cases. It's hard to convice our bench techs to love the Echo with these quirks just yet (they, like most other Blood Bankers expect perfection) but, it won't take long for them to realize that the sensitivity is better than our manual method, and the bigger point, our manual teating will be reduced significantly reducing stress in our busy blood bank.