Ardele- We use manual gel, tubes with PEG as a backup, or tubes with no enhancement. I do this pointless comparison of all three methods we use. Gel is more sensitive than tubes/PEG, and PEG is more sensitive than tubes with no enhancement, so when I get 2+ in Gel, 1+ with PEG, and w+ or neg with no enhancement, I just write "correlates as expected". Really this is a complete waste of time because we all know how the different methods should compare to each other, but I do it anyway so we don't get dinged for our CAP inspections. Hope this helps you out.