lyla_n

We have two ways of testing bacterial contamination of platelets : the Bactalert and other is Verax PGD test. We use them both in conjunction. I would like to know what is the best procedure and ideal process flow of this. Can some one please share their policy for the same.

When a platelet unit tests positive what to do about the other components from same unit. Do we discard them?

thanks.. Here we have a policy of incubating the patients plasma with cord cells and adult O cells at 4 C.. is this the correct way? Is there a standard procedure and protocol for doing cold agglutination testing?

is it a must to do cold agglutinin for every Cardiac surgery or CABG patient? What is the aim of doing this cold agglutinin?

Thats another problem.. The unit was mis labelled as B+. On rechecking the group it was found to be A+ And yes the grouping was done from segment attched to blood bag. - - - Updated - - - No didn do an immediate spin. No H/O bone marrow transplant. And QC of machine and reagents was done

Recently encountered a case of a B+ patient who when cross matched with A+ blood is compatible. the grouping for both the samples was run on Tango as well as Techno and also done manually. No discrepancy in forward and reverse typing. The cross match was also repeated manually. Still compatible. What could be the reasons? patient is a case of CBD stricture scheduled for surgery.Historical blood group is also B+ and no H/O transfusions in the recent past.

O neg 70 y/o , M trauma case. Antibody screen neg. Crossmatched 12 O pos PRBCs , all showing 1+ to 2+ in gel Then cross matched O neg units, compatible. What could be the reason? Why did the antibody screen not show up any thing? We are using Bio rad antibody screening panels

yes that is certainly preferable. But what about when a significant proportion of the donor population is G6 PD deficient?

what is the policy regarding G 6PD def donors?? should they be allowed to donate red cells?or is it better to defer them from blood donation?

Patient 24 y/m b thall DAT 3+ Antibodies: Anti K and anti E transfusion interval shortening progressively to now approx 5 days Current Hb 5g/dl How can we find whether he has developed any other alloantibody? we are currently giving him Phenotypically matched blood for Rh and Kell only. what should be the plan?

How much plasma can be collected from a donor by Trima depending on his weight? Are there any guidelines? Searched the fda site. Cant find it. can some plz post here.

well we did contact the local caridian bct rep and he told us that we dont need saline replacement.. can u tell me from where can i get the specifications as to what should be the volume of a double red cell product? we have configured it to 230ml of each split and saline replacement for >500ml? can u give me a ref for double red cell quality criteria.

if a donor has a hb of 12.5 but his Hct<38. Do we accept him or not as a donor for regular donation and what about plateletpheresis donation? Is it important to have the Hct above 38?

Could any one post a SOP for double red cell collection !!! It would really help.. or from where i could get the guide lines

Double red cell collection: What is the protocol?? Is it necessary to have saline compensation during or after the procedure? Do we have to add any storage solution to the red cell units after collection?? The machine we intend to use is TRIMA accel..